Evidence map›Paper›PMID 33268927›Full record

ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2020

Development of transcriptome-wide SSR markers for genetic diversity and structure analysis in

Rahul Kumar, Sumeet Parkash Kaundal, Vikas Sharma, Ashutosh Sharma, Gagandeep Singh, Ram Kumar Sharma, Rakesh Kumar Chahota, Tilak Raj Sharma

Abstract read
In one paragraph

Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Rahul KumarDAV University, Jalandhar, Punjab, India.
Sumeet Parkash KaundalDAV University, Jalandhar, Punjab, India.
Vikas SharmaSant Baba Bhag Singh University, Khiala, Jalandhar, Punjab, India.ORCID 0000-0002-7378-557X
Ashutosh SharmaDAV University, Jalandhar, Punjab, India.
Gagandeep SinghCentral University of Himachal Pradesh, Dharamshala (H.P), India.
Ram Kumar SharmaCSIR-Institute of Himalayan Bioresource Technology, Palampur (H.P), India.
Rakesh Kumar ChahotaCSKHP Agriculture University, Palampur (H.P), India.
Tilak Raj SharmaICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Horsegram is an important drought resistant pulse crop from Fabaceae and can be easily grown in dry lands with no irrigation facilities. However, it remained neglected since long and has been considered as orphan legume which requires immediate attention for its improvement and for the development of new promising varieties in future. In the present study, 7352 simple sequence repeat (SSR) markers were developed from the transcriptome data and 150 SSR were randomly synthesized for validation and diversity analysis in a panel of 58 horsegram genotypes. The synthesized primers included all types of repeats spanning direpeats to hexarepeats. Of the validated SSR markers, 33 markers were polymorphic and produced 40 loci which were used to analyze the genetic diversity and structure of horsegram. In total, 130 alleles were produced in a range of 2-9 alleles with maximum alleles produced by primer HTSSR 155. Expected heterozygosity (

Indexed as

Genetic diversityGenetic structureHosregramSimple sequence repeats (SSRs)Transcriptome

Identifiers

PMID33268927
PMCPMC7688872

What Socratic holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.