Evidence map›Paper›PMID 33272245›Full record

ArticleBMC cancer2020

MNAT1 promotes proliferation and the chemo-resistance of osteosarcoma cell to cisplatin through regulating PI3K/Akt/mTOR pathway.

Chensheng Qiu, Weiliang Su, Nana Shen, Xiaoying Qi, Xiaolin Wu, Kai Wang, Lin Li, Zhu Guo, Hao Tao, Guanrong Wang and 2 more

Open access · goldAbstract read
In one paragraph

Article in BMC cancer, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 35 citations in OpenAlex.

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  12. The mMolecular cancer · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Chensheng QiuDepartment of Orthopedic Surgery, Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Weiliang SuDepartment of Orthopedic Surgery, Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Nana ShenDepartment of Rehabilitation, Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Xiaoying QiDepartment of Gynaecology, Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Xiaolin WuDepartment of Orthopedic Surgery, Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Kai WangDepartment of Orthopedic Surgery, Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Lin LiDepartment of Rehabilitation, Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Zhu GuoDepartment of Orthopedic Surgery, Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Hao TaoDepartment of Orthopedic Surgery, Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Guanrong WangDepartment of Operation Room, Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Bohua ChenDepartment of Orthopedic Surgery, Affiliated Hospital of Qingdao University, Qingdao, 266000, China. bhchen@hotmail.com.
Hongfei XiangDepartment of Orthopedic Surgery, Affiliated Hospital of Qingdao University, Qingdao, 266000, China. ymdx2004@vip.qq.com.ORCID http://orcid.org/0000-0002-5026-5918
Qingdao University · CN

Funding

National Key Research and Development Project(CN) 2019YFC0121404National Natural Science Foundation of China 81802190,81772412Qingdao Basic Applied Research Project 19-6-2-51-cgthe Young Taishan Scholars Program tsqn201909190
6 · The paper itself

Abstract

backgroundMNAT1 (menage a trois 1, MAT1), a cyclin-dependent kinase-activating kinase (CAK) complex, highly expressed in diverse cancers and was involved in cancer molecular pathogenesis. However, its deliverance profile and biological function in osteosarcoma (OS) remain unclear.

methodsThe expression of MNAT1 in OS was detected by western blot (WB) and immunohistochemistry (IHC). The potential relationship between MNAT1 molecular level expression and OS clinical expectations were analyzed according to tissues microarray (TMA). Proliferation potential of OS cells was evaluated in vitro based on CCK8 and OS cells colony formation assays, while OS cells transwell and in situ tissue source wound healing assays were employed to analyze the OS cells invasion and migration ability in vitro. A nude mouse xenograft model was used to detect tumor growth in vivo. In addition, ordinary bioinformatics analysis and experimental correlation verification were performed to investigate the underlying regulation mechanism of OS by MNAT1.

resultsIn this research, we found and confirmed that MNAT1 was markedly over-expressed in OS tissue derived in situ, also, highly MNAT1 expression was closely associated with bad clinical expectations. Functional studies had shown that MNAT1 silencing could weaken the invasion, migration and proliferation of OS cells in vitro, and inhibit OS tumor growth in vivo. Mechanism study indicated that MNAT1 contributed to the progression of OS via the PI3K/Akt/mTOR pathway. We further verified that the MNAT1 was required in the regulation of OS chemo-sensitivity to cisplatin (DDP).

conclusionsTaken together, the data of the present study demonstrate a novel molecular mechanism of MNAT1 involved in the formation of DDP resistance of OS cells.

Indexed as

AnimalsCell Cycle ProteinsCell ProliferationCisplatinCyclin-Dependent Kinase-Activating KinaseHumansMaleMiceOsteosarcomaPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktTranscription FactorsTransfectionCell Cycle ProteinsCisplatinCyclin-Dependent Kinase-Activating KinaseMNAT1 protein, humanPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktTranscription FactorsCisplatinMNAT1Osteosarcoma

Identifiers

PMID33272245
PMCPMC7713032
OpenAlexW3108483387

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.