Evidence map›Paper›PMID 33294365›Full record

ArticleInternational journal for parasitology. Parasites and wildlife2020

Species-level identification of trypanosomes infecting Australian wildlife by High-Resolution Melting - Real Time Quantitative Polymerase Chain Reaction (HRM-qPCR).

S Keatley, A Botero, J Fosu-Nyarko, L Pallant, A Northover, R C A Thompson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal for parasitology. Parasites and wildlife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Molecular Detection ofPathogens (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

S KeatleyCollege of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, Western Australia, 6150, Australia.
A BoteroCollege of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, Western Australia, 6150, Australia.
J Fosu-NyarkoCollege of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, Western Australia, 6150, Australia.
L PallantCollege of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, Western Australia, 6150, Australia.
A NorthoverCollege of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, Western Australia, 6150, Australia.
R C A ThompsonCollege of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, Western Australia, 6150, Australia.
Murdoch University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Conventional nested PCR and Sanger sequencing methods are currently the gold standards for detecting trypanosomes in wildlife. However, these techniques are time-consuming and can often overlook mixed infections. True trypanosome prevalence can thus be underrepresented. Here, we designed an 18S rDNA-based real-time quantitative PCR (qPCR) assay coupled with High-Resolution Melting Analysis (HRMA) to detect and discriminate three

Indexed as

Australian wildlifeBrushtail possumHigh-resolution melting PCRMarsupialsSYTO 9TrypanosomesWoylie

Identifiers

PMID33294365
PMCPMC7691731
OpenAlexW3101871670

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.