ArticleHearing research2021
Novel 3D-printed hollow microneedles facilitate safe, reliable, and informative sampling of perilymph from guinea pigs.
Article in Hearing research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 78 citations in OpenAlex.
- Physics-Informed Optimization for the Sub-Feature-Scale Fabrication of Hollow Microneedles via Digital Light Processing.Micromachines · 2026Article
- Integrating Polymeric 3D-Printed Microneedles with Wearable Devices: Toward Smart and Personalized Healthcare Solutions.Polymers · 2025Review
- Microneedle-mediated intracochlear injection safely achieves higher perilymphatic dexamethasone concentration than intratympanic delivery in guinea pig.Drug delivery and translational research · 2025Article
- 3D nanoprinting of embryo microinjection needles with anti-clogging features.Microsystems & nanoengineering · 2025Article
- Polymeric 3D-Printed Microneedle Arrays for Non-Transdermal Drug Delivery and Diagnostics.Polymers · 2025Review
- Hollow microneedles as a flexible dosing control solution for transdermal drug delivery.Materials today. Bio · 2025Review
- Programmable NIR Responsive Nanocomposite Enables Noninvasive Intratympanic Delivery of Dexamethasone to Reverse Cisplatin Induced Hearing Loss.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- 3D printed microneedles: revamping transdermal drug delivery systems.Drug delivery and translational research · 2025Review
- Advancements in Materials for 3D-Printed Microneedle Arrays: Enhancing Performance and Biocompatibility.Micromachines · 2024Review
- Exploring Inner Ear and Brain Connectivity through Perilymph Sampling for Early Detection of Neurological Diseases: A Provocative Proposal.Brain sciences · 2024Review
- Direct laser writing-enabled 3D printing strategies for microfluidic applications.Lab on a chip · 2024Review
- Design and fabrication of customizable microneedles enabled by 3D printing for biomedical applications.Bioactive materials · 2024Review
- Formulating biopharmaceuticals using three-dimensional printing.Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques · 2024Review
- Customized flexible hollow microneedles for psoriasis treatment with reduced-dose drug.Bioengineering & translational medicine · 2023Article
- Three Dimensional Printing and Its Applications Focusing on Microneedles for Drug Delivery.Pharmaceutics · 2023Review
- 3D-Printed Microinjection Needle Arrays via a Hybrid DLP-Direct Laser Writing Strategy.Advanced materials technologies · 2023Article
- Microneedle-based interstitial fluid extraction for drug analysis: Advances, challenges, and prospects.Journal of pharmaceutical analysis · 2023Review
- Flexible Monitoring, Diagnosis, and Therapy by Microneedles with Versatile Materials and Devices toward Multifunction Scope.Research (Washington, D.C.) · 2023Review
- A Review of 3D-Printing of Microneedles.Pharmaceutics · 2022Review
- Pyrolyzed Ultrasharp Glassy Carbon Microneedles.Advanced engineering materials · 2022Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
backgroundInner ear diagnostics is limited by the inability to atraumatically obtain samples of inner ear fluid. The round window membrane (RWM) is an attractive portal for accessing perilymph samples as it has been shown to heal within one week after the introduction of microperforations. A 1 µL volume of perilymph is adequate for proteome analysis, yet the total volume of perilymph within the scala tympani of the guinea pig is limited to less than 5 µL. This study investigates the safety and reliability of a novel hollow microneedle device to aspirate perilymph samples adequate for proteomic analysis.
methodsThe guinea pig RWM was accessed via a postauricular surgical approach. 3D-printed hollow microneedles with an outer diameter of 100 µm and an inner diameter of 35 µm were used to perforate the RWM and aspirate 1 µL of perilymph. Two perilymph samples were analyzed by liquid chromatography-mass spectrometry-based quantitative proteomics as part of a preliminary study. Hearing was assessed before and after aspiration using compound action potential (CAP) and distortion product otoacoustic emissions (DPOAE). RWMs were harvested 72 h after aspiration and evaluated for healing using confocal microscopy.
resultsThere was no permanent damage to hearing at 72 h after perforation as assessed by CAP (n = 7) and DPOAE (n = 8), and all perforations healed completely within 72 h (n = 8). In the two samples of perilymph analyzed, 620 proteins were detected, including the inner ear protein cochlin, widely recognized as a perilymph marker.
conclusionHollow microneedles can facilitate aspiration of perilymph across the RWM at a quality and volume adequate for proteomic analysis without causing permanent anatomic or physiologic dysfunction. Microneedles can mediate safe and effective intracochlear sampling and show great promise for inner ear diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.