Evidence map›Paper›PMID 33315191›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D.

Rodrigo A Somoza, Jean F Welter

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Isolation and Culturing of Newborn Rat Nasal Chondrocytes.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Rodrigo A SomozaDepartment of Biology, Skeletal Research Center, Case Center for Multimodal Evaluation of Engineered Cartilage, Case Western Reserve University, Cleveland, OH, USA.
Jean F WelterDepartment of Biology, Skeletal Research Center, Case Center for Multimodal Evaluation of Engineered Cartilage, Case Western Reserve University, Cleveland, OH, USA. jean.welter@case.edu.

Funding

TR&D-4: Growing Tissue in the Scalable, Modular, Automated, and Closed (SMAC) FoundryP41EB021911 · NIBIB · CASE WESTERN RESERVE UNIVERSITY · PI JEAN F WELTER · 2016 to 2026
$13.9M
Total Joint ResurfacingP01AR053622 · NIAMS · CASE WESTERN RESERVE UNIVERSITY · PI MANSOUR, JOSEPH M · 2008 to 2012
$6.0M
Engineering cartilage: an approach to joint repair.R01AR050208 · NIAMS · CASE WESTERN RESERVE UNIVERSITY · PI WELTER, JEAN F · 2005 to 2009
$1.6M
NIAMS NIH HHS P01 AR053622NIAMS NIH HHS R01 AR050208NIBIB NIH HHS P41 EB021911
6 · The paper itself

Abstract

Chondrocytes are the only cell type in cartilage. The dense cartilage extracellular matrix surrounding the chondrocytes makes isolating these cells a complex and lengthy task that subjects the cells to harsh conditions. Protocols to isolate expand and maintain these cells have been improved over the years, providing ways to obtain viable cells for tissue engineering and clinical applications. Here we describe a method to obtain populations of chondrocytes that are able to expand and maintain a native-like phenotype.

Indexed as

Cell Culture TechniquesCell SeparationCartilage, ArticularCells, CulturedChondrocytesHumansSpheroids, CellularCartilage ECMHuman chondrocytesSequential digestion

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.