ArticleFrontiers in nutrition2020
High-Fat Diet Alters the Expression of Reference Genes in Male Mice.
Article in Frontiers in nutrition, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- A Systematic Review of Proteomics in Obesity: Unpacking the Molecular Puzzle.Current obesity reports · 2024Pooled it
- Validation of Optimal Reference Genes for qRT-PCR in Adipose- and Uterine-Derived Feline Mesenchymal Stem Cells.Veterinary sciences · 2026Article
- Selection of appropriate reference genes in ApoeIBRO neuroscience reports · 2026Article
- Modulatory Role of Moringa Oleifera-Loaded Silver Nanoparticles on UCP1 and PPARGC1A Genes Expression in an Obesity Rat Model.Applied biochemistry and biotechnology · 2026Article
- Integrated transcriptomic and 16S rRNA analyses reveal colon and brain barrier-preserving effects of red radish (Raphanus sativus L.) sprout supplementation in high-fat diet-fed mice.Genes & genomics · 2026Article
- Normalization Challenges Across Adipocyte Differentiation and Lipid-Modulating Treatments: Identifying Reliable Housekeeping Genes.International journal of molecular sciences · 2026Article
- Article
- Co-Delivery of Ca-MOF and Mg-MOF Using Nanoengineered Hydrogels to Promote In Situ Mineralization and Bone Defect Repair: In Vitro and In Vivo Analysis.Advanced healthcare materials · 2025Article
- Integrated proteomics and metabolomics elucidate HSD17B12 regulation of intramuscular fat deposition for enhanced beef quality.Food chemistry. Molecular sciences · 2025Article
- Immuno-nutritional therapy in experimental autoimmune encephalomyelitis: a translational pathway to multiple sclerosis management.Inflammopharmacology · 2025Review
- Adipose-specific HuR deletion protects against high-fat diet-induced obesity in mice through upregulating Ucp1 expression.Lipids in health and disease · 2025Article
- Tuberculosis Patients' Serum Extracellular Vesicles Induce Relevant Immune Responses for Initial Defense Against BCG in Mice.Microorganisms · 2025Article
- NIR-Responsive ZIF-8 Metal-Organic Framework Nanohybrids with Photothermal, Antimicrobial, and Osteoinductive Properties to Prevent Implant Infection.Macromolecular bioscience · 2025Article
- Activation of TRPA1 prevents metabolic dysfunction-associated steatotic liver disease in diet-induced obese mice through stimulating the AMPK/CPT1A signaling pathway.Journal of physiology and biochemistry · 2025Article
- TNF-α/NF-κB mediated upregulation of Dectin-1 in hyperglycemic obesity: implications for metabolic inflammation and diabetes.Journal of translational medicine · 2025Article
- Evaluation of reference genes for qPCR in human liver and kidney tissue from individuals with obesity.Scientific reports · 2025Article
- Identification of stable housekeeping genes in mouse liver for studying carbon tetrachloride-induced injury and cellular senescence.Scientific reports · 2024Article
- Long-Term Exposure to Polystyrene Microspheres and High-Fat Diet-Induced Obesity in Mice: Evaluating a Role for Microbiota Dysbiosis.Environmental health perspectives · 2024Article
- Identification of a circulating long non-coding RNA signature panel in plasma as a novel biomarker for the detection of acute/early-stage HIV-1 infection.Biomarker research · 2024Article
- Exercise attenuates high-fat diet-induced PVAT dysfunction through improved inflammatory response and BMP4-regulated adipose tissue browning.Frontiers in nutrition · 2024Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative PCR (qPCR), the most accurate and sensitive technique for quantifying mRNA expression, and choice of appropriate reference genes for internal error controlling in qPCR are essential to understanding the molecular mechanisms that drive the obesity epidemic and its comorbidities. In this study, using the high-fat diet (HFD)-induced obese mouse model, we assessed the expression of 10 commonly used reference genes to validate gene-expression stability in adipose tissue, liver, and muscle across different time points (4, 8, 12, and 16 weeks after HFD feeding) during the process of obesity. The data were analyzed by the GeNorm, NormFinder, BestKeeper, and Delta-Ct method, and the results showed that the most stable reference genes were different for a specific organ or tissue in a specific time point; however, PPIA, RPLP0, and YWHAZ were the top three most stable reference genes in qPCR experiments on adipose, hepatic tissues, and muscles of mice in diet-induced obesity. In addition, the mostly used genes ACTB and GAPDH were more unstable in the fat and liver, the ACTB mRNA levels were increased in four adipose tissues, and the GAPDH mRNA levels were decreased in four adipose tissues and liver after HFD feeding. These results suggest that PPIA, RPLP0, or YWHAZ may be more appropriate to be used as reference gene than ACTB and GAPDH in the adipose tissue and liver of mice during the process of high-fat diet-induced obesity.
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