ArticleNature biomedical engineering2021
A fluorescence sandwich immunoassay for the real-time continuous detection of glucose and insulin in live animals.
Article in Nature biomedical engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed.
- MetaboSense: An Integrated Quantum Dot-Mediated Aptamer Assay and Modular Microfluidic Platform for Continuous In-Line Monitoring of Lung Metabolism During Ex Vivo Perfusion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- An Energy Autonomous Microneedle Array-Based Sensing System for Continuous Biomarker Monitoring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Insulin Detection Enabled by Sensors Employing Fluorescence Signal Amplification Strategy.Luminescence : the journal of biological and chemical luminescence · 2026Article
- Systematic functional screening of switchable aptamer beacon probes.Nature biomedical engineering · 2026Article
- A transistor-based point-of-care assay with lipid-capped sensory interface for clinical profiling of cardiovascular diseases.National science review · 2026Article
- Photothermal Recycling Biosensing for Continuous, Sensitive Molecular Quantification.bioRxiv : the preprint server for biology · 2026Article
- Patient-specific deep offline artificial pancreas for blood glucose regulation in type 1 diabetes.Smart health (Amsterdam, Netherlands) · 2026Article
- Continuous insulin monitoring using an antibody-protecting zwitterionic microneedle patch.Nature biomedical engineering · 2026Article
- Online affinity micro free-flow electrophoresis for the continuous monitoring of insulinLab on a chip · 2026Article
- A ratiometric fluorescent dual-mode amplification nanosensor based on pH and oxygen testing for ultrasensitive glucose detection.Mikrochimica acta · 2026Article
- A refined phenomenological model of viscoelastic clot formation and lysis in trauma-induced coagulopathy.Frontiers in cardiovascular medicine · 2026Article
- Diabetes and postoperative cognitive dysfunction and delirium in adults: mechanisms, biomarkers, and clinical management.Frontiers in endocrinology · 2026Review
- Identifying and minimizing primary sources of temporal broadening in online affinity micro free-flow electrophoresis.The Analyst · 2025Article
- A Small-Molecule Mitofusin 1 Agonist Enhances Islet Survival Under Hypoxic Conditions In Vitro and Improves Transplantation Outcomes.Biomolecules · 2025Article
- From reactive to proactive: Continuous protein monitoring for preventive health care.Science (New York, N.Y.) · 2025Review
- A Bead-Based Quantum Dot Immunoassay Integrated with Multi-Module Microfluidics Enables Real-Time Multiplexed Detection of Blood Insulin and Glucagon.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Engineered single chain variable fragments (scFvs) with improved pH-dependent kinetics for use in continuous biosensor applications.RSC advances · 2025Article
- Metallic, carbon-based, and polymeric nanomaterials: transforming dairy farming practices for sustainability.Food chemistry: X · 2025Review
- Implantable bioelectronics and wearable sensors for kidney health and disease.Nature reviews. Nephrology · 2025Review
- Toward Automated DNA Nanoprinting: Advancing the Synthesis of Covalently Branched DNA.Small methods · 2025Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
Biosensors that continuously measure circulating biomolecules in real time could provide insights into the health status of patients and their response to therapeutics. But biosensors for the continuous real-time monitoring of analytes in vivo have only reached nanomolar sensitivity and can measure only a handful of molecules, such as glucose and blood oxygen. Here we show that multiple analytes can be continuously and simultaneously measured with picomolar sensitivity and sub-second resolution via the integration of aptamers and antibodies into a bead-based fluorescence sandwich immunoassay implemented in a custom microfluidic chip. After an incubation time of 30 s, bead fluorescence is measured using a high-speed camera under spatially multiplexed two-colour laser illumination. We used the assay for continuous quantification of glucose and insulin concentrations in the blood of live diabetic rats to resolve inter-animal differences in the pharmacokinetic response to insulin as well as discriminate pharmacokinetic profiles from different insulin formulations. The assay can be readily modified to continuously and simultaneously measure other blood analytes in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.