Evidence map›Paper›PMID 33355449›Full record

ArticleAnalytical chemistry2021

High-Temporal-Resolution smFISH Method for Gene Expression Studies in

Seleipiri Charles, Guillaume Aubry, Han-Ting Chou, Annalise B Paaby, Hang Lu

Open access · greenAbstract read
In one paragraph

Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Whole-organism spatial transcriptomics at single-cell resolution inbioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Analysis of developmental gene expression using smFISH andbioRxiv : the preprint server for biology · 2024
    Article
  4. Article
  5. Review
  6. Single-molecule FISH inmicroPublication biology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Seleipiri CharlesWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive NW, Atlanta, Georgia 30332, United States.
Guillaume AubrySchool of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive NW, Atlanta, Georgia 30332, United States.
Han-Ting ChouSchool of Biological Sciences, Georgia Institute of Technology, 310 Ferst Drive NW, Atlanta, Georgia 30332, United States.ORCID 0000-0002-1259-2694
Annalise B PaabySchool of Biological Sciences, Georgia Institute of Technology, 310 Ferst Drive NW, Atlanta, Georgia 30332, United States.
Hang LuWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive NW, Atlanta, Georgia 30332, United States.ORCID 0000-0002-6881-660X
Georgia Institute of Technology · US

Funding

Systems variation underlying the genetics of agingR01AG056436 · NIA · UNIVERSITY OF OREGON · PI LU, HANG, PHILLIPS, PATRICK C. · 2017 to 2021
$2.7M
Functional Analysis of Whole-Brain Dynamics in LearningR01NS115484 · NINDS · HARVARD UNIVERSITY · PI LU, HANG, ZHANG, YUN · 2020 to 2024
$2.4M
Quantitative microscopy-based rapid phenotyping and screeningR01GM088333 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI LU, HANG · 2011 to 2018
$2.3M
Genetic Architecture and Developmental Consequences of Conditionally Functional MutationsR35GM119744 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI PAABY, ANNALISE BLOSS · 2016 to 2020
$1.8M
Quantitative Understanding of Mechnosensory Circuit on ChipR01NS096581 · NINDS · GEORGIA INSTITUTE OF TECHNOLOGY · PI LU, HANG · 2016 to 2020
$1.7M
Droplet-based platform for functional neuronal imaging of developing nervous systemR21NS117066 · NINDS · GEORGIA INSTITUTE OF TECHNOLOGY · PI AUBRY, GUILLAUME, LU, HANG · 2020 to 2020
$407k
NIA NIH HHS R01 AG056436NIGMS NIH HHS R01 GM088333NIGMS NIH HHS R35 GM119744NINDS NIH HHS R01 NS096581NINDS NIH HHS R01 NS115484NINDS NIH HHS R21 NS117066
6 · The paper itself

Abstract

Recent development in fluorescence-based molecular tools has contributed significantly to developmental studies, including embryogenesis. Many of these tools rely on multiple steps of sample manipulation, so obtaining large sample sizes presents a major challenge as it can be labor-intensive and time-consuming. However, large sample sizes are required to uncover critical aspects of embryogenesis, for example, subtle phenotypic differences or gene expression dynamics. This problem is particularly relevant for single-molecule fluorescence in situ hybridization (smFISH) studies in

Indexed as

In Situ Hybridization, FluorescenceAnimalsCaenorhabditis elegansEmbryonic DevelopmentEmbryo, Nonmammalian

Identifiers

PMID33355449
PMCPMC10619480
OpenAlexW3114231583

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.