Evidence map›Paper›PMID 33359883›Full record

ArticleTuberculosis (Edinburgh, Scotland)2021

Proteomic profile of vitreous in patients with tubercular uveitis.

Reema Bansal, Mohd M Khan, Surendra Dasari, Indu Verma, David R Goodlett, Nathan P Manes, Aleksandra Nita-Lazar, Surya P Sharma, Aman Kumar, Nirbhai Singh and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tuberculosis (Edinburgh, Scotland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 2 countries.

Reema BansalAdvanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: drreemab@rediffmail.com.
Mohd M KhanUniversity of Maryland, School of Medicine, Baltimore, MD, USA; Laboratory of Immune System Biology (LISB), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA. Electronic address: mohsin.khan@umaryland.edu.
Surendra DasariDepartment of Health Sciences Research, Mayo Clinic, Rochester, MN, USA. Electronic address: Dasari.Surendra@mayo.edu.
Indu VermaDepartment of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: induvermabio@gmail.com.
David R GoodlettUniversity of Maryland, Baltimore, MD, USA. Electronic address: Goodlett.laboratory@gmail.com.
Nathan P ManesLaboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. Electronic address: nathan.manes@nih.gov.
Aleksandra Nita-LazarLaboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. Electronic address: nitalazarau@niaid.nih.gov.
Surya P SharmaAdvanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: sharmapsurya@gmail.com.
Aman KumarAdvanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: aman.jain1424@gmail.com.
Nirbhai SinghAdvanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: nirbhais@gmail.com.
Anuradha ChakrabortiDepartment of Experimental Medicine & Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: superoxide.14@gmail.com.
Vishali GuptaAdvanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: vishalisara@yahoo.co.in.
M R DograAdvanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: drmangatdogra@gmail.com.
Jagat RamAdvanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: drjagatram@gmail.com.
Amod GuptaAdvanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: dramodgupta@gmail.com.
Post Graduate Institute of Medical Education and Research · INNational Institutes of Health · USUniversity of Maryland, Baltimore · USMayo Clinic in Florida · US

Funding

Intramural NIH HHS Z99 AI999999
6 · The paper itself

Abstract

objectiveTo elucidate disease-specific host protein profile in vitreous fluid of patients with intraocular inflammation due to tubercular uveitis (TBU).

methodsVitreous samples from 13 patients with TBU (group A), 7 with non-TBU (group B) and 9 with no uveitis (group C) were analysed by shotgun proteomics using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Differentially expressed proteins (DEPs) were subjected to pathway analysis using WEB-based Gene SeT Analysis Toolkit software.

resultsCompared to control groups (B + C combined), group A (TBU) displayed 32 (11 upregulated, 21 downregulated) DEPs, which revealed an upregulation of coagulation cascades, complement and classic pathways, and downregulation of metabolism of carbohydrates, gluconeogenesis, glucose metabolism and glycolysis/gluconeogenesis pathways. When compared to group B (non-TBU) alone, TBU displayed 58 DEPs (21 upregulated, 37 downregulated), with an upregulation of apoptosis, KRAS signaling, diabetes pathways, classic pathways, and downregulation of MTORC1 signaling, glycolysis/gluconeogenesis, and glucose metabolism.

conclusionThis differential protein profile provides novel insights into the molecular mechanisms of TBU and a baseline to explore vitreous biomarkers to differentiate TBU from non-TBU, warranting future studies to identify and validate them as a diagnostic tool in TBU. The enriched pathways generate interesting hypotheses and drive further research.

Indexed as

AdolescentAdultAgedBiomarkersCase-Control StudiesChromatography, LiquidFemaleHumansMaleMiddle AgedMycobacterium tuberculosisProteomeProteomicsTuberculosis, OcularUveitisVitreous BodyBiomarkersProteomeExtrapulmonary tuberculosisShotgun proteomicsTubercular uveitisTuberculosisUveitisVitreous

Identifiers

PMID33359883
PMCPMC11005023
OpenAlexW3107669562

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.