Evidence mapPaperPMID 33372152Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2021

PACAP is a pathogen-inducible resident antimicrobial neuropeptide affording rapid and contextual molecular host defense of the brain.

Ernest Y Lee, Liana C Chan, Huiyuan Wang, Juelline Lieng, Mandy Hung, Yashes Srinivasan, Jennifer Wang, James A Waschek, Andrew L Ferguson, Kuo-Fen Lee and 3 more

Open access · bronzeAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 1 pooled it
3.4field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Review
  6. SARS-CoV-2 peptide fragments selectively dysregulate specific immune cell populations via Gaussian curvature targeting.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  7. Article
  8. Review
  9. Article
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  11. Novel technologies uncover novel 'anti'-microbial peptides inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024
    Article
  12. Article
  13. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 1 country.

Ernest Y LeeDepartment of Bioengineering, University of California, Los Angeles, CA 90095.ORCID 0000-0001-5144-2552
Liana C ChanLundquist Institute for Biomedical Innovation, Harbor-UCLA Medical Center, Torrance, CA 90509.ORCID 0000-0003-2567-4418
Huiyuan WangLundquist Institute for Biomedical Innovation, Harbor-UCLA Medical Center, Torrance, CA 90509.
Juelline LiengDepartment of Bioengineering, University of California, Los Angeles, CA 90095.
Mandy HungDepartment of Bioengineering, University of California, Los Angeles, CA 90095.
Yashes SrinivasanDepartment of Bioengineering, University of California, Los Angeles, CA 90095.ORCID 0000-0002-5279-9753
Jennifer WangDepartment of Bioengineering, University of California, Los Angeles, CA 90095.
James A WaschekSemel Institute for Neuroscience and Human Behavior, Intellectual Development and Disabilities Research Center, David Geffen School of Medicine, University of California, Los Angeles, CA 90095.
Andrew L FergusonPritzker School of Molecular Engineering, University of Chicago, Chicago, IL 60637.ORCID 0000-0002-8829-9726
Kuo-Fen LeePeptide Biology Laboratories, Salk Institute for Biological Studies, La Jolla, CA 92037.ORCID 0000-0003-2224-2708
Nannette Y YountLundquist Institute for Biomedical Innovation, Harbor-UCLA Medical Center, Torrance, CA 90509.ORCID 0000-0002-7079-5211
Michael R YeamanDivision of Molecular Medicine, Los Angeles County, Harbor-UCLA Medical Center, Torrance, CA 90509; MRYeaman@ucla.edu gclwong@seas.ucla.edu.
Gerard C L WongDepartment of Bioengineering, University of California, Los Angeles, CA 90095; MRYeaman@ucla.edu gclwong@seas.ucla.edu.ORCID 0000-0003-0893-6383
University of California, Los Angeles · USUCLA Medical Center · USIntel (United States) · USCalifornia Institute of Technology · USCalifornia NanoSystems Institute · USSalk Institute for Biological Studies · USUniversity of Chicago · US

Funding

Viral Vector Core (VVC)P30CA014195 · NCI · SALK INSTITUTE FOR BIOLOGICAL STUDIES · 1985 to 2025
$37.5M
MEDICAL SCIENTIST TRAINING PROGRAMT32GM008042 · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1985 to 2005
$6.0M
Cathelicidins in Skin ImmunityR01AI052453 · VETERANS MEDICAL RESEARCH FDN/SAN DIEGO · 2002 to 2005
$1.1M
Institutional Career Development CoreKL2TR001882 · UNIVERSITY OF CALIFORNIA LOS ANGELES · 2025 to 2025
$1.0M
NIGMS SYSTEMS &INTEGRATIVE BIOLOGYT32GM008185 · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1987 to 2005
$911k
Surface sensing, memory, and motility control in biofilm formationR01AI143730 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI George A. O'Toole, GERARD C WONG · 2022 to 2023
$778k
NCATS NIH HHS KL2 TR001882NCI NIH HHS P30 CA014195NIAID NIH HHS R01 AI052453NIAID NIH HHS R01 AI143730NIAID NIH HHS R21 AI111661NIAID NIH HHS R33 AI111661NIAID NIH HHS U01 AI124319NIAMS NIH HHS T32 AR071307NIA NIH HHS R56 AG062232NIA NIH HHS RF1 AG054714NIA NIH HHS RF1 AG064049NIGMS NIH HHS P41 GM103393NIGMS NIH HHS T32 GM008042NIGMS NIH HHS T32 GM008185NIH HHS R24 OD023076NIMH NIH HHS U19 MH114831NINDS NIH HHS R33 NS115183NINDS NIH HHS R61 NS115183
6 · The paper itself

Abstract

Defense of the central nervous system (CNS) against infection must be accomplished without generation of potentially injurious immune cell-mediated or off-target inflammation which could impair key functions. As the CNS is an immune-privileged compartment, inducible innate defense mechanisms endogenous to the CNS likely play an essential role in this regard. Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide known to regulate neurodevelopment, emotion, and certain stress responses. While PACAP is known to interact with the immune system, its significance in direct defense of brain or other tissues is not established. Here, we show that our machine-learning classifier can screen for immune activity in neuropeptides, and correctly identified PACAP as an antimicrobial neuropeptide in agreement with previous experimental work. Furthermore, synchrotron X-ray scattering, antimicrobial assays, and mechanistic fingerprinting provided precise insights into how PACAP exerts antimicrobial activities vs. pathogens via multiple and synergistic mechanisms, including dysregulation of membrane integrity and energetics and activation of cell death pathways. Importantly, resident PACAP is selectively induced up to 50-fold in the brain in mouse models of

Indexed as

Amino Acid SequenceAnimalsAnti-Infective AgentsAntimicrobial Cationic PeptidesBrainCell DeathComputer SimulationDatabases, GeneticInflammationMiceMice, Inbred BALB CNeuropeptidesPhylogenyPituitary Adenylate Cyclase-Activating PolypeptideSignal TransductionAnti-Infective AgentsAntimicrobial Cationic PeptidesNeuropeptidesPituitary Adenylate Cyclase-Activating Polypeptideantimicrobial peptideshost defenseinnate immunityneuroimmunologyneuropeptides

Identifiers

PMID33372152
PMCPMC7817161
OpenAlexW3115031845

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.