Evidence map›Paper›PMID 33383000›Full record

ArticleDevelopmental cell2021

Chromosome Segregation and Peptidoglycan Remodeling Are Coordinated at a Highly Stabilized Septal Pore to Maintain Bacterial Spore Development.

Ahmed M T Mohamed, Helena Chan, Johana Luhur, Elda Bauda, Benoit Gallet, Cécile Morlot, Louise Cole, Milena Awad, Simon Crawford, Dena Lyras and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Synergistic potential of LeuBMC microbiology · 2024
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Extracellular Vesicles of the Plant PathogenJournal of fungi (Basel, Switzerland) · 2023
    Article
  10. Article
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ahmed M T MohamedThe ithree Institute, University of Technology Sydney (UTS), Sydney NSW, Australia.
Helena ChanThe ithree Institute, University of Technology Sydney (UTS), Sydney NSW, Australia.
Johana LuhurThe ithree Institute, University of Technology Sydney (UTS), Sydney NSW, Australia.
Elda BaudaUniversity of Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France.
Benoit GalletUniversity of Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France.
Cécile MorlotUniversity of Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France.
Louise ColeThe ithree Institute, University of Technology Sydney (UTS), Sydney NSW, Australia; Microbial Imaging Facility (MIF), University of Technology Sydney (UTS), Sydney NSW, Australia.
Milena AwadInfection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Melbourne VIC, Australia.
Simon CrawfordRamaciotti Centre for Cryo-Electron Microscopy, Monash University, Melbourne VIC, Australia.
Dena LyrasInfection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Melbourne VIC, Australia.
David Z RudnerDepartment of Microbiology, Harvard Medical School, Boston, MA, USA.
Christopher D A RodriguesThe ithree Institute, University of Technology Sydney (UTS), Sydney NSW, Australia. Electronic address: christopher.rodrigues@uts.edu.au.

Funding

Chromosome Dynamics in Bacillus SubtillsR01GM086466 · NIGMS · HARVARD MEDICAL SCHOOL · PI RUDNER, DAVID Z · 2009 to 2021
$4.2M
Cell Envelope Homeostasis in Bacillus subtilisR01GM127399 · NIGMS · HARVARD MEDICAL SCHOOL · PI RUDNER, DAVID Z · 2019 to 2022
$1.4M
NIGMS NIH HHS R01 GM086466NIGMS NIH HHS R01 GM127399
6 · The paper itself

Abstract

Asymmetric division, a hallmark of endospore development, generates two cells, a larger mother cell and a smaller forespore. Approximately 75% of the forespore chromosome must be translocated across the division septum into the forespore by the DNA translocase SpoIIIE. Asymmetric division also triggers cell-specific transcription, which initiates septal peptidoglycan remodeling involving synthetic and hydrolytic enzymes. How these processes are coordinated has remained a mystery. Using Bacillus subtilis, we identified factors that revealed the link between chromosome translocation and peptidoglycan remodeling. In cells lacking these factors, the asymmetric septum retracts, resulting in forespore cytoplasmic leakage and loss of DNA translocation. Importantly, these phenotypes depend on septal peptidoglycan hydrolysis. Our data support a model in which SpoIIIE is anchored at the edge of a septal pore, stabilized by newly synthesized peptidoglycan and protein-protein interactions across the septum. Together, these factors ensure coordination between chromosome translocation and septal peptidoglycan remodeling to maintain spore development.

Indexed as

Chromosome SegregationBacillus subtilisBacterial ProteinsCell WallChromosomesMicroscopy, Electron, TransmissionPenicillin-Binding ProteinsPeptidoglycanPeriplasmic ProteinsProtein BindingSpores, BacterialBacterial ProteinsPbpG protein, Bacillus subtilisPenicillin-Binding ProteinsPeptidoglycanPeriplasmic Proteinsspore-specific proteins, Bacilluscell wallchromosome segregationchromosome translocationdevelopmentendosporespeptidoglycanSpoIIIEsporessporulation

Identifiers

PMID33383000
PMCPMC8048138

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.