Evidence map›Paper›PMID 33404286›Full record

ArticleRNA biology2021

Identification and initial characterization of POLIII-driven transcripts by msRNA-sequencing.

Peter Zorn, Danny Misiak, Michael Gekle, Marcel Köhn

Abstract read
In one paragraph

Article in RNA biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Peter ZornUniversity of Halle-Wittenberg, Germany.
Danny MisiakInstitute of Molecular Medicine, University of Halle-Wittenberg, Halle (Saale), Germany.ORCID 0000-0001-5931-8425
Michael GekleJulius-Bernstein-Institute of Physiology, University of Halle-Wittenberg, Germany.
Marcel KöhnUniversity of Halle-Wittenberg, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Non-coding RNAs (ncRNAs) are powerful regulators of gene expression but medium-sized (50-300 nts in length) ncRNAs (msRNAs) are barely picked-up precisely by RNA-sequencing. Here we describe msRNA-sequencing (msRNAseq), a modified protocol that associated with a computational analyses pipeline identified about ~1800 msRNA loci, including over 300 putatively novel msRNAs, in human and murine cells. We focused on the identification and initial characterization of three POLIII-derived transcripts. The validation of these uncharacterized msRNAs identified an ncRNA in antisense orientation from the

Indexed as

HumansParaspecklesRNA, AntisenseRNA, MessengerRNA Polymerase IIIRNA, UntranslatedSequence Analysis, RNAPOLR3E protein, humanRNA, AntisenseRNA, MessengerRNA Polymerase IIIRNA, UntranslatedMsrnancRNApolarpoliiipolr3e

Identifiers

PMID33404286
PMCPMC8583065

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.