Evidence mapPaperPMID 33407572Full record

ArticleReproductive biology and endocrinology : RB&E2021

Association of three missense mutations in the homocysteine-related MTHFR and MTRR gene with risk of polycystic ovary syndrome in Southern Chinese women.

Wanqin Feng, Yan Zhang, Yuan Pan, Yi Zhang, Minjuan Liu, Yuxin Huang, Yuanling Xiao, Wenyu Mo, Junjie Jiao, Xiaoyang Wang and 3 more

Abstract read
In one paragraph

Article in Reproductive biology and endocrinology : RB&E, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  7. Unraveling Extremely DamagingJournal of personalized medicine · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Wanqin FengDepartment of Gynecology, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, No.253, Gongye Middle Avenue, Haizhu District, 510280, Guangzhou, Guangdong, China.
Yan ZhangClinical Research Center, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
Yuan PanDepartment of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
Yi ZhangDepartment of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
Minjuan LiuDepartment of Gynecology, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, No.253, Gongye Middle Avenue, Haizhu District, 510280, Guangzhou, Guangdong, China.
Yuxin HuangDepartment of Obstetrics, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
Yuanling XiaoDepartment of Obstetrics, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
Wenyu MoDepartment of Gynecology, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, No.253, Gongye Middle Avenue, Haizhu District, 510280, Guangzhou, Guangdong, China.
Junjie JiaoDepartment of Fetal Medicine and Prenatal Diagnosis, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
Xiaoyang WangDepartment of Gynecology, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, No.253, Gongye Middle Avenue, Haizhu District, 510280, Guangzhou, Guangdong, China.
Dan TianDepartment of Obstetrics, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
Lixia YangDepartment of Emergency, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
Ying MaDepartment of Gynecology, Obstetrics and Gynecology Center, Zhujiang Hospital, Southern Medical University, No.253, Gongye Middle Avenue, Haizhu District, 510280, Guangzhou, Guangdong, China. mayingwuzhuoyi@126.com.ORCID http://orcid.org/0000-0003-1856-101X

Funding

National Natural Science Foundation of China 81701418
6 · The paper itself

Abstract

backgroundThe etiology between homocysteine and polycystic ovary syndrome (PCOS) is unclear. In humans, the level of homocysteine is mainly affected by two enzymes: methylene tetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR). While the activity of these two enzymes is mainly affected by three missense mutations, namely C677T (MTHFR), A1298C (MTHFR), and A66G (MTRR). This study aims to examine the association between the three missense mutations and PCOS and investigate whether the three missense mutations exerted their effect on PCOS by affecting the homocysteine level.

methodsA case-control study was designed, comprising 150 people with PCOS and 300 controls. Logistic regression analysis was used to assess the association between the three missense mutations and PCOS. Linear regression analysis was used to assess the association between the three missense mutations and the homocysteine level. Mediation analysis was used to investigate whether the three missense mutations exerted their effect on PCOS by affecting the homocysteine level.

resultsFollowing adjustments and multiple rounds of testing, MTHFR A1298C was found to be significantly associated with PCOS in a dose-dependent manner (compared to AA, OR = 2.142 for AC & OR = 3.755 for CC; P < 0.001). MTRR A66G was nominally associated with PCOS. Mutations in MTHFR A1298C and MTRR A66G were significantly associated with the homocysteine level. Mediation analysis suggested the effect of MTHFR A1298C on PCOS was mediated by homocysteine.

conclusionsMTHFR A1298C and MTRR A66G were associated with PCOS, and MTHFR A1298C might affect the risk of PCOS by influencing the homocysteine level.

Indexed as

Mutation, MissenseAdultAsian PeopleCase-Control StudiesChinaFemaleFerredoxin-NADP ReductaseGene FrequencyGenetic Predisposition to DiseaseGenotypeHomocysteineHumansLinkage DisequilibriumMethylenetetrahydrofolate Reductase (NADPH2)Polycystic Ovary SyndromePolymorphism, Single NucleotideFerredoxin-NADP ReductaseHomocysteinemethionine synthase reductaseMethylenetetrahydrofolate Reductase (NADPH2)Folic acidHomocysteineMTHFRMTRRPolycystic ovary syndrome

Identifiers

PMID33407572
PMCPMC7789417

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.