Evidence mapPaperPMID 33413331Full record

ArticleBMC complementary medicine and therapies2021

Suppression of autophagy through JAK2/STAT3 contributes to the therapeutic action of rhynchophylline on asthma.

Hui Li, Qianyu Bi, Hongxia Cui, Chuanfeng Lv, Meng Wang

Open access · goldAbstract read
In one paragraph

Article in BMC complementary medicine and therapies, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
5.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Hui LiDepartment of Medical Affairs, Jining No. 1 People's Hospital, Jiankang Road, Jining, Shandong, 272011, People's Republic of China.
Qianyu BiInstitute of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, 250355, People's Republic of China.
Hongxia CuiDepartment of Oncology, Jining No. 1 People's Hospital, Jining, Shandong, 272011, People's Republic of China.
Chuanfeng LvDepartment of Pharmacology, Jining No. 1 People's Hospital, Jining, Shandong, 272011, People's Republic of China.
Meng WangDepartment of Medical Affairs, Jining No. 1 People's Hospital, Jiankang Road, Jining, Shandong, 272011, People's Republic of China. wangmeng106@163.com.ORCID http://orcid.org/0000-0001-7830-5216
Jining First People's Hospital · CNShandong University of Traditional Chinese Medicine · CN

Funding

Key Research and Development Plan of Jining City 2019SMNS003Key Research and Development Plan of Shandong Province 2019GSF108172Natural Science Foundation of Shandong Province (CN) 2016ZRA08005Traditional Chinese Medicine Science and Technology Development Project of Shandong Province 2015-443Wu Jieping Medical Foundation 320.6750.2020-04-8
6 · The paper itself

Abstract

backgroundAsthma is a chronic inflammatory disease characterized by airway remodeling and inflammation. Rhynchophylline is a kind of indole alkaloid isolated from Uncaria rhynchophylla. Here we investigated the effect of rhynchophylline on autophagy in asthma.

methodsA mice model of asthma was established by ovalbumin challenge. Histopathological changes were assessed by hematoxylin-eosin staining, periodic acid-schiff staining and Masson staining. The levels of IgE in serum, interleukin-6 and interleukin-13 in bronchoalveolar lavage fluid, as well as the activities of superoxide dismutase and catalase in lung tissues were detected. The expression of autophagy-related genes and Janus kinase (JAK) 2/ signal transducer and activator of transcription (STAT) 3 signal was detected by western blot and immunofluorescence. Airway smooth muscle cells (ASMCs) were isolated, and the effect rhynchophylline on autophagy in ASMCs was explored.

resultsOur data showed that rhynchophylline treatment alleviated inflammation, airway remodeling, and oxidative stress in asthma. In addition, autophagy, which was implicated in asthma, was suppressed by rhynchophylline with decreased level of autophagy-related proteins. Furthermore, rhynchophylline suppressed the JAK2/STAT3 signaling pathway, which was activated in asthma. In vitro study showed that rhynchophylline suppressed ASMC autophagy through suppressing the activation of JAK2/STAT3 signal.

conclusionsOur study demonstrated that rhynchophylline can alleviate asthma through suppressing autophagy in asthma, and that JAK2/STAT3 signal was involved in this effect of rhynchophylline. This study indicates that rhynchophylline may become a promising drug for the treatment of asthma.

Indexed as

Airway RemodelingAnimalsAnti-Asthmatic AgentsAnti-Inflammatory Agents, Non-SteroidalAsthmaFemaleJanus Kinase 2MiceMice, Inbred BALB COxidative StressOxindolesSignal TransductionSTAT3 Transcription FactorUncariaAnti-Asthmatic AgentsAnti-Inflammatory Agents, Non-SteroidalJak2 protein, mouseJanus Kinase 2OxindolesrhyncophyllineStat3 protein, mouseSTAT3 Transcription FactorAsthmaAutophagyJAK2RhynchophyllineSTAT3

Identifiers

PMID33413331
PMCPMC7792286
OpenAlexW3118712093

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.