Evidence map›Paper›PMID 33413611›Full record

ArticleBiotechnology for biofuels2021

Production of 10-methyl branched fatty acids in yeast.

Hannah G Blitzblau, Andrew L Consiglio, Paulo Teixeira, Donald V Crabtree, Shuyan Chen, Oliver Konzock, Gamuchirai Chifamba, Austin Su, Annapurna Kamineni, Kyle MacEwen and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. Optimization ofMicroorganisms · 2023
    Article
  4. Article
  5. Lipid Readjustment inBiomolecules · 2022
    Article
  6. Article
  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 4 countries.

Hannah G BlitzblauNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA. hblitzblau@ginkgobioworks.com.ORCID http://orcid.org/0000-0001-6830-2652
Andrew L ConsiglioNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA.
Paulo TeixeiraDepartment of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden.
Donald V CrabtreeNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA.
Shuyan ChenNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA.
Oliver KonzockDepartment of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden.
Gamuchirai ChifambaNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA.
Austin SuNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA.
Annapurna KamineniNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA.
Kyle MacEwenNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA.
Maureen HamiltonNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA.
Vasiliki TsakraklidesNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA.
Jens NielsenDepartment of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden.
Verena SiewersDepartment of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden.
A Joe ShawNovogy, Inc., 85 Bolton Street, Cambridge, MA, 02140, USA.
Ginkgo Bioworks, Inc. (United States) · USChalmers University of Technology · SENovo Nordisk Foundation · DKEuropean Bioinformatics Institute · GBManus Bio (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDespite the environmental value of biobased lubricants, they account for less than 2% of global lubricant use due to poor thermo-oxidative stability arising from the presence of unsaturated double bonds. Methyl branched fatty acids (BFAs), particularly those with branching near the acyl-chain mid-point, are a high-performance alternative to existing vegetable oils because of their low melting temperature and full saturation.

resultsWe cloned and characterized two pathways to produce 10-methyl BFAs isolated from actinomycetes and γ-proteobacteria. In the two-step bfa pathway of actinomycetes, BfaB methylates Δ9 unsaturated fatty acids to form 10-methylene BFAs, and subsequently, BfaA reduces the double bond to produce a fully saturated 10-methyl branched fatty acid. A BfaA-B fusion enzyme increased the conversion efficiency of 10-methyl BFAs. The ten-methyl palmitate production (tmp) pathway of γ-proteobacteria produces a 10-methylene intermediate, but the TmpA putative reductase was not active in E. coli or yeast. Comparison of BfaB and TmpB activities revealed a range of substrate specificities from C14-C20 fatty acids unsaturated at the Δ9, Δ10 or Δ11 position. We demonstrated efficient production of 10-methylene and 10-methyl BFAs in S. cerevisiae by secretion of free fatty acids and in Y. lipolytica as triacylglycerides, which accumulated to levels more than 35% of total cellular fatty acids.

conclusionsWe report here the characterization of a set of enzymes that can produce position-specific methylene and methyl branched fatty acids. Yeast expression of bfa enzymes can provide a platform for the large-scale production of branched fatty acids suitable for industrial and consumer applications.

Indexed as

10-Methylstearic acidBiobased lubricantTuberculostearic acidYarrowia lipolytica

Identifiers

PMID33413611
PMCPMC7791843
OpenAlexW3119909118

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.