Evidence map›Paper›PMID 33420117›Full record

ArticleScientific reports2021

Extracellular vesicle-associated repetitive element DNAs as candidate osteosarcoma biomarkers.

Linda Cambier, Kevin Stachelek, Martin Triska, Rima Jubran, Manyu Huang, Wuyin Li, Jianying Zhang, Jitian Li, David Cobrinik

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 2 pooled it
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 37 citations in OpenAlex.

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  12. Exosomes: a promising avenue for cancer diagnosis beyond treatment.Frontiers in cell and developmental biology · 2024
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  15. Effect of radiotherapy on the DNA cargo and cellular uptake mechanisms of extracellular vesicles.Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al] · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Linda CambierThe Vision Center and The Saban Research Institute, Children's Hospital Los Angeles, 4650 Sunset Blvd, MS163, Los Angeles, CA, 90027, USA.
Kevin StachelekThe Vision Center and The Saban Research Institute, Children's Hospital Los Angeles, 4650 Sunset Blvd, MS163, Los Angeles, CA, 90027, USA.
Martin TriskaThe Vision Center and The Saban Research Institute, Children's Hospital Los Angeles, 4650 Sunset Blvd, MS163, Los Angeles, CA, 90027, USA.
Rima JubranDivision of Hematology/Oncology, Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA.
Manyu HuangHenan Luoyang Orthopedic Hospital (Henan Provincial Orthopedic Hospital)/Henan Provincial Orthopedic Institute, 82 Qiming Road, Luoyang, 471002, Henan, China.
Wuyin LiHenan Luoyang Orthopedic Hospital (Henan Provincial Orthopedic Hospital)/Henan Provincial Orthopedic Institute, 82 Qiming Road, Luoyang, 471002, Henan, China.
Jianying ZhangDepartment of Biological Science, The University of Texas at El Paso, El Paso, TX, 79968, USA.
Jitian LiHenan Luoyang Orthopedic Hospital (Henan Provincial Orthopedic Hospital)/Henan Provincial Orthopedic Institute, 82 Qiming Road, Luoyang, 471002, Henan, China. jitianlee@hotmail.com.
David CobrinikThe Vision Center and The Saban Research Institute, Children's Hospital Los Angeles, 4650 Sunset Blvd, MS163, Los Angeles, CA, 90027, USA. dcobrinik@chla.usc.edu.
Children's Hospital of Los Angeles · USLuoyang Orthopedic-Traumatological Hospital of Henan Province · CNThe University of Texas at El Paso · USUniversity of Southern California · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Osteosarcoma (OS) is the most common malignant bone tumor in children and young adults. Despite that high-risk factors have been identified, no test for early detection is available. This study aimed to identify circulating nucleic acid sequences associated with serum extracellular vesicle (EV) preparations at the time of OS diagnosis, as a step towards an OS early detection assay. Sequencing of small nucleic acids extracted from serum EV preparations revealed increased representation of diverse repetitive element sequences in OS patient versus control sera. Analysis of a validation cohort using qPCR of PEG-precipitated EV preparations revealed the over-representation of HSATI, HSATII, LINE1-P1, and Charlie 3 at the DNA but not RNA level, with receiver operating characteristic (ROC) area under the curve (AUC) ≥ 0.90. HSATI and HSATII DNAs co-purified with EVs prepared by precipitation and size exclusion chromatography but not by exosome immunocapture, indicative of packaging in a non-exosomal complex. The consistent over-representation of EV-associated repetitive element DNA sequences suggests their potential utility as biomarkers for OS and perhaps other cancers.

Indexed as

AdolescentAdultBiomarkersBone NeoplasmsChildChild, PreschoolDNAExtracellular VesiclesFemaleHumansMaleOsteosarcomaRepetitive Sequences, Nucleic AcidYoung AdultBiomarkersDNA

Identifiers

PMID33420117
PMCPMC7794510
OpenAlexW3120219493

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.