ArticleMicrobial biotechnology2021
Integration of dsRNA against host immune response genes augments the virulence of transgenic Metarhizium robertsii strains in insect pest species.
Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Article
- Effects ofJournal of fungi (Basel, Switzerland) · 2026Article
- Acetylation dynamics of MrATG4 governing autophagy-mediated conidiation in an entomopathogenic fungus.PLoS pathogens · 2026Article
- Enhancing the Virulence of a Fungal Entomopathogen Against the Brown Planthopper by Expressing dsRNA to Suppress Host Immune Defenses.Microorganisms · 2025Article
- Engineered fungus containing a caterpillar gene kills insects rapidly by disrupting their ecto- and endo-microbiomes.Communications biology · 2024Article
- A novel partitivirus orchestrates conidiation, stress response, pathogenicity, and secondary metabolism of the entomopathogenic fungus Metarhizium majus.PLoS pathogens · 2023Article
- A life-and-death struggle: interaction of insects with entomopathogenic fungi across various infection stages.Frontiers in immunology · 2023Review
- Infection with a novel polymycovirus enhances growth, conidiation and sensitivity to UV-B irradiation of the entomopathogenic fungusFrontiers in microbiology · 2023Article
- Functional Analysis of Keto-Acid Reductoisomerase ILVC in the Entomopathogenic FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Integration of dsRNA against host immune response genes augments the virulence of transgenic Metarhizium robertsii strains in insect pest species.Microbial biotechnology · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The slow lethality of fungal biopesticides to insects restrains their widespread application as a strategy of pest control. In this study, unary, binary and ternary transgenic Metarhizium robertsii were created by integrating genes that encode the scorpion neurotoxin BjαIT, the cuticle-degrading protease Pr1A, and a double-stranded RNA (dsRNA) that targets host gnbp3, individually or collectively under a constitutive promoter to enhance virulence. Compared with the parental wild type, all unary transgenic strains had increased virulence against four insect species, Tenebrio molitor, Locusta migratoria, Plutella xylostella and Galleria mellonella, whereas the binary transgenic strain expressing both pr1A and BjαIT had increased virulence to T. molitor and L. migratoria, with no change in virulence against P. xylostella and G. mellonella. Importantly, all ternary transgenic strains simultaneously expressing pr1A, BjαIT, and the dsRNA specific to host gnbp3 exhibited the highest increase in insect-specific virulence. This finding highlights a novel strategy for genetic engineering of dsRNAs that target genes associated with the host immune response alongside virulence genes to maximize fungal virulence and lethality against insect pests.
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