ArticleNature protocols2021
Characterization of connective tissues using near-infrared spectroscopy and imaging.
Article in Nature protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 74 citations in OpenAlex.
- Second Harmonic Generation Imaging Links Collagen Structure and Biochemical Composition in Human Osteoarthritic Cartilage.Annals of biomedical engineering · 2026Article
- The Relationship Between the Optical Properties of Articular Cartilage and the Biomechanical Parameters of the Tissue.Journal of biophotonics · 2025Article
- The Multifactorial Relationship Between Bone Tissue Water and Stiffness at the Proximal Femur.Calcified tissue international · 2025Article
- Noninvasive Optical Sensing of Aging and Diet Preferences Using Raman Spectroscopy.Analytical chemistry · 2025Article
- Infrared Spectroscopy Can Differentiate Between Cartilage Injury Models: Implication for Assessment of Cartilage Integrity.Annals of biomedical engineering · 2024Article
- Holistic vibrational spectromics assessment of human cartilage for osteoarthritis diagnosis.Biomedical optics express · 2024Article
- Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications.Bone research · 2024Review
- Non-neotissue constituents as underestimated confounders in the assessment of tissue engineered constructs by near-infrared spectroscopy.Materials today. Bio · 2024Article
- Towards a point-of-care multimodal spectroscopy instrument for the evaluation of human cardiac tissue.Heart and vessels · 2023Article
- Broadband scattering properties of articular cartilage zones and their relationship with the heterogenous structure of articular cartilage extracellular matrix.Journal of biomedical optics · 2023Article
- Point-of-care detection of fibrosis in liver transplant surgery using near-infrared spectroscopy and machine learning.Health science reports · 2023Article
- Raman spectroscopy-based prediction of ofloxacin concentration in solution using a novel loss function and an improved GA-CNN model.BMC bioinformatics · 2023Article
- Recent Advances in Biomimetic Nanocarrier-Based Photothermal Therapy for Cancer Treatment.International journal of molecular sciences · 2023Review
- Impact of experimental setup parameters on the measurement of articular cartilage optical properties in the visible and short near-infrared spectral bands.Biomedical optics express · 2023Article
- Open-sourced Raman spectroscopy data processing package implementing a baseline removal algorithm validated from multiple datasets acquired in human tissue and biofluids.Journal of biomedical optics · 2023Article
- Assessment of Ligament Viscoelastic Properties Using Raman Spectroscopy.Annals of biomedical engineering · 2022Article
- Article
- Resolving the Near-Infrared Spectrum of Articular Cartilage.Cartilage · 2021Article
- A Tissue Section-Based Near-Infrared Spectroscopical Analysis of Salivary Gland Tumors.Cancers · 2021Article
- Articular cartilage optical properties in the near-infrared (NIR) spectral range vary with depth and tissue integrity.Biomedical optics express · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Near-infrared (NIR) spectroscopy is a powerful analytical method for rapid, non-destructive and label-free assessment of biological materials. Compared to mid-infrared spectroscopy, NIR spectroscopy excels in penetration depth, allowing intact biological tissue assessment, albeit at the cost of reduced molecular specificity. Furthermore, it is relatively safe compared to Raman spectroscopy, with no risk of laser-induced photothermal damage. A typical NIR spectroscopy workflow for biological tissue characterization involves sample preparation, spectral acquisition, pre-processing and analysis. The resulting spectrum embeds intrinsic information on the tissue's biomolecular, structural and functional properties. Here we demonstrate the analytical power of NIR spectroscopy for exploratory and diagnostic applications by providing instructions for acquiring NIR spectra, maps and images in biological tissues. By adapting and extending this protocol from the demonstrated application in connective tissues to other biological tissues, we expect that a typical NIR spectroscopic study can be performed by a non-specialist user to characterize biological tissues in basic research or clinical settings. We also describe how to use this protocol for exploratory study on connective tissues, including differentiating among ligament types, non-destructively monitoring changes in matrix formation during engineered cartilage development, mapping articular cartilage proteoglycan content across bovine patella and spectral imaging across the depth-wise zones of articular cartilage and subchondral bone. Depending on acquisition mode and experiment objectives, a typical exploratory study can be completed within 6 h, including sample preparation and data analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.