Evidence map›Paper›PMID 33467856›Full record

ArticleJournal of proteome research2021

Use of Hybrid Data-Dependent and -Independent Acquisition Spectral Libraries Empowers Dual-Proteome Profiling.

Patrick Willems, Ursula Fels, An Staes, Kris Gevaert, Petra Van Damme

Abstract read
In one paragraph

Article in Journal of proteome research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Opsonization Improves Dual Proteomic Analysis ofJournal of proteome research · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Patrick WillemsDepartment of Biochemistry and Microbiology, Ghent University, Ghent 9000, Belgium.ORCID 0000-0003-4667-2294
Ursula FelsDepartment of Biochemistry and Microbiology, Ghent University, Ghent 9000, Belgium.
An StaesVIB-UGent Center for Medical Biotechnology, Ghent 9052, Belgium.
Kris GevaertVIB-UGent Center for Medical Biotechnology, Ghent 9052, Belgium.ORCID 0000-0002-4237-0283
Petra Van DammeDepartment of Biochemistry and Microbiology, Ghent University, Ghent 9000, Belgium.ORCID 0000-0001-9090-027X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the context of bacterial infections, it is imperative that physiological responses can be studied in an integrated manner, meaning a simultaneous analysis of both the host and the pathogen responses. To improve the sensitivity of detection, data-independent acquisition (DIA)-based proteomics was found to outperform data-dependent acquisition (DDA) workflows in identifying and quantifying low-abundant proteins. Here, by making use of representative bacterial pathogen/host proteome samples, we report an optimized hybrid library generation workflow for DIA mass spectrometry relying on the use of data-dependent and

Indexed as

ProteomeProteomicsMass SpectrometryPeptidesWorkflowPeptidesProteomebacterial pathogen/host interactiondata-dependent acquisition (DDA)data-independent acquisition (DIA)Salmonellaspectral library

Identifiers

PMID33467856
PMCPMC7871992

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.