ArticleCommunications biology2021
AAV1 is the optimal viral vector for optogenetic experiments in pigeons (Columba livia).
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Chemogenetic activation of the amygdala core nucleus increases aggression in chickens.iScience · 2026Article
- Achieving cell-type specific transduction with adeno-associated viral vectors in pigeons.Current research in neurobiology · 2026Article
- Anesthetic and analgesic management of Svalbard rock ptarmigan (Lagopus muta hyperborea) undergoing experimental stereotaxic neurosurgery.BMC veterinary research · 2025Article
- Adeno-associated viruses for efficient gene expression in the axolotl nervous system.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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- Functional MRI of imprinting memory in awake newborn domestic chicks.Communications biology · 2024Article
- Enhancing translation: A need to leverage complex preclinical models of addictive drugs to accelerate substance use treatment options.Pharmacology, biochemistry, and behavior · 2024Review
- Genomic loss of GPR108 disrupts AAV transduction in birds.bioRxiv : the preprint server for biology · 2024Article
- Long-term, high-resolution in vivo calcium imaging in pigeons.Cell reports methods · 2024Article
- Balanced anesthesia in pigeons (Frontiers in physiology · 2024Article
- A custom-made AAV1 variant (AAV1-T593K) enables efficient transduction of Japanese quail neurons in vitro and in vivo.Communications biology · 2023Article
- Article
- Visual categories and concepts in the avian brain.Animal cognition · 2023Review
- Molecular biology of serotonergic systems in avian brains.Frontiers in molecular neuroscience · 2023Review
- Dose-response relationship between the variables of unilateral optogenetic stimulation and transcallosal evoked responses in rat motor cortex.Frontiers in neuroscience · 2022Article
- Article
- Genetically identified neurons in avian auditory pallium mirror core principles of their mammalian counterparts.Current biology : CB · 2021Article
- Red Light Optogenetics in Neuroscience.Frontiers in cellular neuroscience · 2021Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although optogenetics has revolutionized rodent neuroscience, it is still rarely used in other model organisms as the efficiencies of viral gene transfer differ between species and comprehensive viral transduction studies are rare. However, for comparative research, birds offer valuable model organisms as they have excellent visual and cognitive capabilities. Therefore, the following study establishes optogenetics in pigeons on histological, physiological, and behavioral levels. We show that AAV1 is the most efficient viral vector in various brain regions and leads to extensive anterograde and retrograde ChR2 expression when combined with the CAG promoter. Furthermore, transient optical stimulation of ChR2 expressing cells in the entopallium decreases pigeons' contrast sensitivity during a grayscale discrimination task. This finding demonstrates causal evidence for the involvement of the entopallium in contrast perception as well as a proof of principle for optogenetics in pigeons and provides the groundwork for various other methods that rely on viral gene transfer in birds.
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