ArticlePlant biotechnology journal2021
Improvement of host-induced gene silencing efficiency via polycistronic-tRNA-amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Optimization of dsRNA Length and Target Position for Enhanced RNA Interference Efficiency Targeting the Chitinase GeneInsects · 2026Article
- RNAi: the next wave of green agriculture, challenges, and bridging the translational gap.Plant cell reports · 2026Review
- Identification and RNA Interference-Based Functional Analysis of Chitin Deacetylase Genes inInsects · 2026Article
- Plant RNA interference from antiviral silencing to multiplex trait engineering for climate-resilient crops.Frontiers in plant science · 2026Review
- A Polycistronic tRNA-amiRNA System Reveals the Antiviral Roles ofPlants (Basel, Switzerland) · 2025Article
- Production Technologies for Recombinant Antibodies: Insights into Eukaryotic, Prokaryotic, and Transgenic Expression Systems.Biochemical genetics · 2025Review
- IbPIF1 confers stem nematode resistance by regulating secondary metabolites in sweet potato.Plant biotechnology journal · 2025Article
- Harnessing double-stranded RNA (dsRNA): a sustainable approach to pest management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Transgenic expression of mAChR-C dsRNA in maize confers efficient locust control.Plant communications · 2025Article
- Silencing Multiple Crustacean Hyperglycaemic Hormone-Encoding Genes in the Redclaw CrayfishInternational journal of molecular sciences · 2024Article
- Host-Delivered RNA Interference for Durable Pest Resistance in Plants: Advanced Methods, Challenges, and Applications.Molecular biotechnology · 2024Review
- RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry.Plant cell reports · 2024Article
- Dichotomous sperm in Lepidopteran insects: a biorational target for pest management.Frontiers in insect science · 2023Review
- Host-induced silencing of the CpCHI gene resulted in developmental abnormalities and mortality in maize stem borer (Chilo partellus).PloS one · 2023Article
- FAR knockout significantly inhibits Chilo suppressalis survival and transgene expression of double-stranded FAR in rice exhibits strong pest resistance.Plant biotechnology journal · 2022Article
- Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (International journal of molecular sciences · 2022Article
- Improvement of host-induced gene silencing efficiency via polycistronic-tRNA-amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata.Plant biotechnology journal · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Host-induced gene silencing (HIGS) emerged as a new strategy for pest control. However, RNAi efficiency is reported to be low in Lepidoptera, which are composed of many important crop pests. To address this, we generated transgenic plants to develop HIGS effects in a maize pest, Mythimna separata (Lepidoptera, Noctuidae), by targeting chitinase encoding genes. More importantly, we developed an artificial microRNA (amiR) based PTA (polycistronic-tRNA-amiR) system for silencing multiple target genes. Compared with hpRNA (hairpin RNA), transgenic expression of a PTA cassette including an amiR for the gut-specific dsRNA nuclease gene MsREase, resulted in improved knockdown efficiency and caused more pronounced developmental abnormalities in recipient insects. When target gene siRNAs were analysed after HIGS and direct dsRNA/siRNA feeding, common features such as sense polarity and siRNA hotspot regions were observed, however, they differed in siRNA transitivity and major 20-24nt siRNA species. Core RNAi genes were identified in M. separata, and biochemical activities of MsAGO2, MsSID1 and MsDcr2 were confirmed by EMSA (electrophoretic mobility shift assay) and dsRNA cleavage assays, respectively. Taken together, we provide compelling evidence for the existence of the RNAi mechanism in M. separata by analysis of both siRNA signatures and RNAi machinery components, and the PTA system could potentially be useful for future RNAi control of lepidopteran pests.
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