ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2021
Dbx2 regulation in limbs suggests interTAD sharing of enhancers.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Decoding the DNA of Anatolian water buffalo by genome-wide discoveries for body size and ultrasound carcass traits.BMC veterinary research · 2025Article
- Structural variants in the 3D genome as drivers of disease.Nature reviews. Genetics · 2025Review
- Bystander activation across a TAD boundary supports a cohesin-dependent transcription cluster model for enhancer function.Genes & development · 2025Article
- Genetic factors mediating long-range enhancer-promoter communication in mammalian development.Current opinion in genetics & development · 2025Review
- Article
- Cooperative insulation of regulatory domains by CTCF-dependent physical insulation and promoter competition.Nature communications · 2024Article
- Boundary stacking interactions enable cross-TAD enhancer-promoter communication during limb development.Nature genetics · 2024Article
- Emerging questions on the mechanisms and dynamics of 3D genome evolution in spiralians.Briefings in functional genomics · 2023Review
- A novel chromosome 2q24.3-q32.1 microdeletion in a fetus with multiple malformations.Journal of clinical laboratory analysis · 2022Article
- Context-dependent enhancer function revealed by targeted inter-TAD relocation.Nature communications · 2022Article
- The stochastic nature of genome organization and function.Current opinion in genetics & development · 2022Review
- Article
- Dbx2 regulation in limbs suggests interTAD sharing of enhancers.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- Detecting chromosomal interactions in Capture Hi-C data with CHiCAGO and companion tools.Nature protocols · 2021Article
- Mesomelic dysplasias associated with the HOXD locus are caused by regulatory reallocations.Nature communications · 2021Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDuring tetrapod limb development, the HOXA13 and HOXD13 transcription factors are critical for the emergence and organization of the autopod, the most distal aspect where digits will develop. Since previous work had suggested that the Dbx2 gene is a target of these factors, we set up to analyze in detail this potential regulatory interaction.
resultsWe show that HOX13 proteins bind to mammalian-specific sequences at the vicinity of the Dbx2 locus that have enhancer activity in developing digits. However, the functional inactivation of the DBX2 protein did not elicit any particular phenotype related to Hox genes inactivation in digits, suggesting either redundant or compensatory mechanisms. We report that the neighboring Nell2 and Ano6 genes are also expressed in distal limb buds and are in part controlled by the same Dbx2 enhancers despite being localized into two different topologically associating domains (TADs) flanking the Dbx2 locus.
conclusionsWe conclude that Hoxa13 and Hoxd genes cooperatively activate Dbx2 expression in developing digits through binding to mammalian specific regulatory sequences in the Dbx2 neighborhood. Furthermore, these enhancers can overcome TAD boundaries in either direction to co-regulate a set of genes located in distinct chromatin domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.