Evidence map›Paper›PMID 33531386›Full record

ArticlemBio2021

Genetic Evidence for SecY Translocon-Mediated Import of Two Contact-Dependent Growth Inhibition (CDI) Toxins.

Allison M Jones, Petra Virtanen, Disa Hammarlöf, William J Allen, Ian Collinson, Christopher S Hayes, David A Low, Sanna Koskiniemi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

Allison M JonesDepartment of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA.
Petra VirtanenDepartment of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
Disa HammarlöfDepartment of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
William J AllenSchool of Biochemistry, University of Bristol, Bristol, United Kingdom.ORCID 0000-0002-9513-4786
Ian CollinsonSchool of Biochemistry, University of Bristol, Bristol, United Kingdom.ORCID 0000-0002-3931-0503
Christopher S Hayes *Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA.
David A Low *Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA david.low@lifesci.ucsb.edu.
Sanna Koskiniemi *Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.ORCID 0000-0002-3275-0936
Uppsala University · SEUniversity of Bristol · GBUniversity of California, Santa Barbara · US

Funding

Molecular mechanisms of antibacterial CDI toxin activationR01GM117373 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI GOULDING, CELIA, HAYES, CHRISTOPHER S. · 2016 to 2019
$1.2M
Biotechnology and Biological Sciences Research Council BB/V001531/1NIGMS NIH HHS R01 GM117373
6 · The paper itself

Abstract

The C-terminal (CT) toxin domains of contact-dependent growth inhibition (CDI) CdiA proteins target Gram-negative bacteria and must breach both the outer and inner membranes of target cells to exert growth inhibitory activity. Here, we examine two CdiA-CT toxins that exploit the bacterial general protein secretion machinery after delivery into the periplasm. A Ser281Phe amino acid substitution in transmembrane segment 7 of SecY, the universally conserved channel-forming subunit of the Sec translocon, decreases the cytotoxicity of the membrane depolarizing orphan10 toxin from enterohemorrhagic

Indexed as

Contact InhibitionEscherichia coli ProteinsMembrane ProteinsMutationProtein TransportSEC Translocation ChannelsCdiA protein, E coliEscherichia coli ProteinsMembrane ProteinsSEC Translocation Channelsbacterial competitionmembrane potentialtype V secretiontype V secretion system

Identifiers

PMID33531386
PMCPMC7858069
OpenAlexW3112437208

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.