ArticleBMC microbiology2021
Helicobacter pylori 23S rRNA gene A2142G, A2143G, T2182C, and C2195T mutations associated with clarithromycin resistance detected in Sudanese patients.
Article in BMC microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Clarithromycin-resistant Helicobacter pylori in Africa: a systematic review and meta-analysis.Antimicrobial resistance and infection control · 2025Pooled it
- Association of Resistance-AssociatedAntibiotics (Basel, Switzerland) · 2026Article
- Antimicrobial resistance and virulence patterns ofInfectious medicine · 2026Article
- High Prevalence ofAntibiotics (Basel, Switzerland) · 2025Article
- Towards EffectiveInternational journal of molecular sciences · 2025Review
- A Comprehensive Review of Molecular Mechanisms Leading to the Emergence of Multidrug Resistance in Bacteria.Indian journal of microbiology · 2025Review
- Identification of genetic determinants of antibiotic resistance in Helicobacter pylori isolates in Vietnam by high-throughput sequencing.BMC microbiology · 2025Article
- Prevalence and Variability ofAntibiotics (Basel, Switzerland) · 2025Article
- Detection ofCurrent issues in molecular biology · 2025Article
- Helicobacter pylori infection, clarithromycin-resistant genes and CYP2C19 gene polymorphisms in asymptomatic school-age children in Baigou New Town, Baoding City, Hebei Province.Pakistan journal of medical sciences · 2025Article
- Antimicrobial drug susceptibility testing for the management ofFrontiers in microbiology · 2025Review
- High salt condition alters LPS synthesis and induces the emergence of drug resistance mutations inAntimicrobial agents and chemotherapy · 2024Article
- Genetic Markers ofCurrent issues in molecular biology · 2024Article
- Evidence of Helicobacter pylori heterogeneity in human stomachs by susceptibility testing and characterization of mutations in drug-resistant isolates.Scientific reports · 2024Article
- Research on Key Technologies of Microarray Chips for Detecting Drug-Resistant Genes inMicromachines · 2024Article
- Editorial: Clinical management ofFrontiers in medicine · 2024Article
- Prevalence of Clarithromycin-Resistant Helicobacter pylori Strains in Zambia: A Sub-Saharan African Country.Digestive diseases (Basel, Switzerland) · 2024Article
- A Mini-review onEndocrine, metabolic & immune disorders drug targets · 2024Review
- Strategies ofFrontiers in cellular and infection microbiology · 2024Review
- Polymorphism of virulence genes and biofilm associated with in vitro induced resistance to clarithromycin in Helicobacter pylori.Gut pathogens · 2023Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClarithromycin resistant Helicobacter pylori (H. pylori) strains represent a worldwide health problem. These stains are usually carrying mutations within the 23S rRNA gene associated with clarithromycin resistance. This study aimed to detect H. pylori and clarithromycin resistant associated mutations from Sudanese patients with gastritis symptoms. MATERIALS AND
methodsTwo hundred and eighty-eight gastric biopsies were collected using gastrointestinal endoscopy from patients with gastritis symptoms in different hospitals in Khartoum state. H. pylori was detected by PCR using primer targeting 16S rRNA. Then allele-specific PCR and DNA sequencing were used to screen for the presence of A2142G and A2143G point mutations.
resultsOut of 288 samples, H. pylori was detected in 88 (~ 30.6%) samples by 16 s RNA. Allele-specific PCR detected the variant A2142G in 9/53 (~ 17%) sample, while A2143G mutation was not found in any sample. The DNA sequencing revealed the presence of mutations associated with clarithromycin-resistance in 36% (9/25) of samples; the A2142G was present in one sample, A2143G in 5 samples and T2182C in 4 samples. Additionally, another point mutation (C2195T) was detected in 3 samples. There was no association of 23S rRNA gene point mutations with gender, age group, and patients' geographical distribution.
conclusionThis study revealed a high frequency (36%) of mutations associated with clarithromycin resistance using DNA sequencing of the 23S rRNA gene's V domain. This information should be taken into consideration to avoid eradication therapy failing.
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Registered trials
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