ArticleReproductive biology and endocrinology : RB&E2021
Pretreatment with melatonin improves ovarian tissue cryopreservation for transplantation.
Article in Reproductive biology and endocrinology : RB&E, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Low-Dose Melatonin, Climacteric Symptoms and Sleep in Female Shift Workers: A Randomized Controlled Trial.Journal of pineal research · 2026Trial
- Ovarian puncture alters genes but not follicle growth in cyclophosphamide mice model.Reproduction & fertility · 2026Article
- Melatonin Enhances Glutathione Peroxidase Activity and Improves Antioxidant Defense in Cryopreserved Ovarian Transplants: A Rat Model Study.Antioxidants (Basel, Switzerland) · 2026Article
- Comparative efficacy of melatonin and glutathione in mitigating carboplatin-induced ovarian toxicity in rats.Clinics (Sao Paulo, Brazil) · 2026Article
- Frozen-Thawed ovarian autografts treated with scaffold-based melatonin delivery in rats.Reproductive biology and endocrinology : RB&E · 2026Article
- Identification of cryosensitive niches and a targetable FOS/AP‑1 program in the human ovarian cortex by single‑cell and spatial transcriptomics.BMC medicine · 2026Article
- Article
- Epigallocatechin gallate cooperated with hydrogel encapsulation enables high-performance cryopreservation of mouse ovaries.Materials today. Bio · 2025Article
- Alpha-lipoic acid reduces oxidative damage and ameliorates follicular abnormalities in vitrified cat ovarian tissue.Frontiers in endocrinology · 2025Article
- Effect of Elamipretide on the Vitrification of Mouse Ovarian Tissue by Freezing.Biopreservation and biobanking · 2024Article
- Melatonin increases superoxide dismutase 2 (SOD2) levels and improves rat ovarian graft function after transplantation.Journal of ovarian research · 2024Article
- Antioxidant Actions of Melatonin: A Systematic Review of Animal Studies.Antioxidants (Basel, Switzerland) · 2024Review
- Melatonin, menopause, and thyroid function in gynecologic endocrinology: what is the role?Revista da Associacao Medica Brasileira (1992) · 2024Article
- E-cadherin and NF-κB expression in the vagina after ovarian ischemia and reperfusion.Acta cirurgica brasileira · 2024Article
- Breast cancer survivals and hormone therapy: estrogen and melatonin.Revista da Associacao Medica Brasileira (1992) · 2023Article
- Safe transport of organs and tissues for transplants: technological innovation product validation method.Revista da Associacao Medica Brasileira (1992) · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroudMelatonin has anti-inflammatory and antioxidative actions at the mitochondrial level. This indole-containing molecule may protect ovarian grafts during the process of cryopreservation. Therefore, we aimed to determine whether melatonin pretreatment improves rat ovarian graft quality.
methodsTwenty-six female rats were allocated to two study groups of thirteen animals each: 1) control group: ovaries cryopreserved using the standard protocol; and 2) melatonin group: ovaries cryopreserved in a medium with melatonin. Ten rats of each group were submitted to 24-h freezing, and whole ovaries autologous and avascular transplantation with retroperitoneal placement. After postoperative (PO) day 15, daily vaginal smears were obtained for estrous cycle characterization. Between PO days 30 and 35, the animals were euthanized and ovarian grafts were recovered for histological and immunohistochemical (Ki-67, cleaved caspase-3, TUNEL, von Willebrand factor, estrogen, and progesterone receptors) analyses. The ovaries of the three remaining rats from each group were studied immediately after thawing to assess the effects of cryopreservation. ANOVA and Tukey's tests were used and the rejection level of the null hypothesis was set at 0.05 or 5% (p < 0.05).
resultsMelatonin promoted faster restart of the estrous cycle and increased the expression of mature follicles, collagen type I, von Willebrand factor, Ki-67, and cleaved caspase-3 on corpora lutea and estrogen receptors in the ovaries as compared to control. There was a reduction in apoptosis by TUNEL on follicles, corpora lutea, and collagen type III.
conclusionBased on the evaluated parameters, melatonin may promote the quality of ovarian grafts. Reproductive function enhancement should be further studied.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.