Evidence map›Paper›PMID 33536571›Full record

ArticleScientific reports2021

Arrayed CRISPR reveals genetic regulators of tau aggregation, autophagy and mitochondria in Alzheimer's disease model.

Lishu Duan, Mufeng Hu, Joseph A Tamm, Yelena Y Grinberg, Fang Shen, Yating Chai, Hualin Xi, Lauren Gibilisco, Brinda Ravikumar, Vivek Gautam and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 26 citations in OpenAlex.

  1. CRISPR-Based Gene Therapy for Brain Disease.Molecular neurobiology · 2026
    Review
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  8. CRISPRi: a way to integrate iPSC-derived neuronal models.Biochemical Society transactions · 2024
    Review
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  10. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Lishu DuanCambridge Research Center, AbbVie, 200 Sidney Street, Cambridge, MA, 02139, USA. Lishu.duan@abbvie.com.
Mufeng HuAbbVie Inc., 1 North Waukegan Rd., North Chicago, IL, 60064, USA.
Joseph A TammCambridge Research Center, AbbVie, 200 Sidney Street, Cambridge, MA, 02139, USA.
Yelena Y GrinbergCambridge Research Center, AbbVie, 200 Sidney Street, Cambridge, MA, 02139, USA.
Fang ShenCambridge Research Center, AbbVie, 200 Sidney Street, Cambridge, MA, 02139, USA.
Yating ChaiAbbVie Inc., 1 North Waukegan Rd., North Chicago, IL, 60064, USA.
Hualin XiCambridge Research Center, AbbVie, 200 Sidney Street, Cambridge, MA, 02139, USA.
Lauren GibiliscoAbbVie Inc., 1 North Waukegan Rd., North Chicago, IL, 60064, USA.
Brinda RavikumarCambridge Research Center, AbbVie, 200 Sidney Street, Cambridge, MA, 02139, USA.
Vivek GautamCambridge Research Center, AbbVie, 200 Sidney Street, Cambridge, MA, 02139, USA.
Eric KarranCambridge Research Center, AbbVie, 200 Sidney Street, Cambridge, MA, 02139, USA.
Matthew TownsendCambridge Research Center, AbbVie, 200 Sidney Street, Cambridge, MA, 02139, USA.
Robert V TalanianCambridge Research Center, AbbVie, 200 Sidney Street, Cambridge, MA, 02139, USA.
AbbVie (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alzheimer's disease (AD) is a common neurodegenerative disease with poor prognosis. New options for drug discovery targets are needed. We developed an imaging based arrayed CRISPR method to interrogate the human genome for modulation of in vitro correlates of AD features, and used this to assess 1525 human genes related to tau aggregation, autophagy and mitochondria. This work revealed (I) a network of tau aggregation modulators including the NF-κB pathway and inflammatory signaling, (II) a correlation between mitochondrial morphology, respiratory function and transcriptomics, (III) machine learning predicted novel roles of genes and pathways in autophagic processes and (IV) individual gene function inferences and interactions among biological processes via multi-feature clustering. These studies provide a platform to interrogate underexplored aspects of AD biology and offer several specific hypotheses for future drug discovery efforts.

Indexed as

Models, GeneticAlzheimer DiseaseAutophagyBrainCell Line, TumorCRISPR-Cas SystemsGene Expression RegulationGenetic EngineeringHumansMachine LearningMitochondriaNeuronsProtein Aggregation, PathologicalSignal Transductiontau Proteinstau Proteins

Identifiers

PMID33536571
PMCPMC7859211
OpenAlexW3127012266

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.