ArticlePlant, cell & environment2021
An amiRNA screen uncovers redundant CBF and ERF34/35 transcription factors that differentially regulate arsenite and cadmium responses.
Article in Plant, cell & environment, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- The transcription elongation factor IWS1 promotes thermomorphogenesis by integrating the brassinosteroid and gibberellin pathways in Arabidopsis.Plant communications · 2026Article
- XAP5 CIRCADIAN TIMEKEEPER coordinates circadian rhythms and anthocyanin biosynthesis independently of splicing.Plant physiology · 2026Article
- ChERF017, an ERF transcription factor, confers cadmium stress tolerance in Cerasus humilis.BMC plant biology · 2026Article
- mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis.Plant physiology · 2026Article
- Identification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Metal transport proteins and transcription factor networks in plant responses to cadmium stress.Plant cell reports · 2024Review
- Review
- Transcription factors involved in plant responses to cadmium-induced oxidative stress.Frontiers in plant science · 2024Review
- Probing the physiological role of the plastid outer-envelope membrane using the oemiR plasmid collection.G3 (Bethesda, Md.) · 2023Article
- The TabHLH094-TaMYC8 complex mediates the cadmium response in wheat.Molecular breeding : new strategies in plant improvement · 2023Article
- Article
- Transcriptome analysis and differential expression in Arabidopsis thaliana in response to rohitukine (a chromone alkaloid) treatment.Functional & integrative genomics · 2023Article
- Molecular insight into arsenic uptake, transport, phytotoxicity, and defense responses in plants: a critical review.Planta · 2022Review
- Arsenic perception and signaling: The yet unexplored world.Frontiers in plant science · 2022Review
- The SLIM1 transcription factor is required for arsenic resistance in Arabidopsis thaliana.FEBS letters · 2021Article
- Advances in "Omics" Approaches for Improving Toxic Metals/Metalloids Tolerance in Plants.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
Abstract
Arsenic stress causes rapid transcriptional responses in plants. However, transcriptional regulators of arsenic-induced gene expression in plants remain less well known. To date, forward genetic screens have proven limited for dissecting arsenic response mechanisms. We hypothesized that this may be due to the extensive genetic redundancy present in plant genomes. To overcome this limitation, we pursued a forward genetic screen for arsenite tolerance using a randomized library of plants expressing >2,000 artificial microRNAs (amiRNAs). This library was designed to knock-down diverse combinations of homologous gene family members within sub-clades of transcription factor and transporter gene families. We identified six transformant lines showing an altered response to arsenite in root growth assays. Further characterization of an amiRNA line targeting closely homologous CBF and ERF transcription factors show that the CBF1,2 and 3 transcription factors negatively regulate arsenite sensitivity. Furthermore, the ERF34 and ERF35 transcription factors are required for cadmium resistance. Generation of CRISPR lines, higher-order T-DNA mutants and gene expression analyses, further support our findings. These ERF transcription factors differentially regulate arsenite sensitivity and cadmium tolerance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.