ArticleMethods in molecular biology (Clifton, N.J.)2021
Quantitative Electron Microscopy to Study HCMV Morphogenesis.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- DeepEM Playground: Bringing deep learning to electron microscopy labs.Journal of microscopy · 2025Article
- Secondary Envelopment of Human Cytomegalovirus Is a Fast Process Utilizing the Endocytic Compartment as a Major Membrane Source.Biomolecules · 2024Article
- The baseless mutant links protein phosphatase 2A with basal cell identity in the brown alga Ectocarpus.Development (Cambridge, England) · 2023Article
- Near-Native Visualization of SARS-CoV-2 Induced Membrane Remodeling and Virion Morphogenesis.Viruses · 2022Article
- Weighted average ensemble-based semantic segmentation in biological electron microscopy images.Histochemistry and cell biology · 2022Article
- Role of mutations and post-translational modifications in systemic AL amyloidosis studied by cryo-EM.Nature communications · 2021Article
- A filamentous archaeal virus is enveloped inside the cell and released through pyramidal portals.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The generation and release of mature virions from human cytomegalovirus (HCMV) infected cells is a multistep process, involving a profound reorganization of cellular structures and various stages of virus particle morphogenesis in different cellular compartments. Although the general steps of HCMV morphogenesis are known, it has become clear that the detailed molecular mechanisms are complex and dependent on various viral factors and cellular pathways. The lack of a full understanding of HCMV virion morphogenesis emphasizes the need of imaging techniques to visualize the different stages of virion assembly, such as electron microscopy. Here, we describe various electron microscopy techniques and the methodology of high-pressure freezing and freeze substitution for sample preparation to visualize HCMV morphogenesis. These methods are used in our laboratory in combination with a thorough quantification to characterize phenotypic alterations and to identify the function of viral and cellular proteins for the various morphogenesis stages.
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Registered trials
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