Evidence map›Paper›PMID 33572565›Full record

ArticleInternational journal of molecular sciences2021

A Cellular Assay for the Identification and Characterization of Connexin Gap Junction Modulators.

Azeem Danish, Robin Gedschold, Sonja Hinz, Anke C Schiedel, Dominik Thimm, Peter Bedner, Christian Steinhäuser, Christa E Müller

Open access · goldAbstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Glial connexins in glaucoma.Frontiers in neuroscience · 2025
    Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Azeem DanishPharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical & Medicinal Chemistry, Rheinische Friedrich-Wilhelms-Universität Bonn, An der Immenburg 4, D-53121 Bonn, Germany.
Robin GedscholdPharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical & Medicinal Chemistry, Rheinische Friedrich-Wilhelms-Universität Bonn, An der Immenburg 4, D-53121 Bonn, Germany.
Sonja HinzPharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical & Medicinal Chemistry, Rheinische Friedrich-Wilhelms-Universität Bonn, An der Immenburg 4, D-53121 Bonn, Germany.
Anke C SchiedelPharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical & Medicinal Chemistry, Rheinische Friedrich-Wilhelms-Universität Bonn, An der Immenburg 4, D-53121 Bonn, Germany.ORCID 0000-0002-8114-3139
Dominik ThimmPharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical & Medicinal Chemistry, Rheinische Friedrich-Wilhelms-Universität Bonn, An der Immenburg 4, D-53121 Bonn, Germany.
Peter BednerInstitute of Cellular Neuroscience, Medical Faculty, Rheinische Friedrich-Wilhelms-Universität Bonn, Venusberg-Campus 1, D-53127 Bonn, Germany.
Christian SteinhäuserInstitute of Cellular Neuroscience, Medical Faculty, Rheinische Friedrich-Wilhelms-Universität Bonn, Venusberg-Campus 1, D-53127 Bonn, Germany.ORCID 0000-0003-2579-8357
Christa E MüllerPharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical & Medicinal Chemistry, Rheinische Friedrich-Wilhelms-Universität Bonn, An der Immenburg 4, D-53121 Bonn, Germany.ORCID 0000-0002-0013-6624
University of Bonn · DE

Funding

Bundesministerium für Bildung und Forschung CONNEXIN - 16GW0181K and 16GW0182
6 · The paper itself

Abstract

Connexin gap junctions (Cx GJs) enable the passage of small molecules and ions between cells and are therefore important for cell-to-cell communication. Their dysfunction is associated with diseases, and small molecules acting as modulators of GJs may therefore be useful as therapeutic drugs. To identify GJ modulators, suitable assays are needed that allow compound screening. In the present study, we established a novel assay utilizing HeLa cells recombinantly expressing Cx43. Donor cells additionally expressing the Gs protein-coupled adenosine A

Indexed as

Biosensing TechniquesCarbenoxoloneCell CommunicationConnexin 43Cyclic AMPGap JunctionsHeLa CellsHigh-Throughput Screening AssaysHumansInhibitory Concentration 50LuciferasesCarbenoxoloneConnexin 43Cyclic AMPGJA1 protein, humanLuciferasescompound libraryconnexin-43gap junctionsGloSensor luciferaseHeLa cellsscreening

Identifiers

PMID33572565
PMCPMC7866863
OpenAlexW3127187865

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.