ArticleNature communications2021
Production of germ-free mosquitoes via transient colonisation allows stage-specific investigation of host-microbiota interactions.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 110 citations in OpenAlex.
- Crosstalk in the cold: host-microbe interactions in insect diapause.Annals of the Entomological Society of America · 2026Review
- Article
- Maternal instincts: a review of the role of chemosensory cues and larval habitat conditions on oviposition site choice by gravid Aedes aegypti (Diptera: Culicidae).Journal of medical entomology · 2026Review
- Dismissing-then-recruiting: a unique mechanism for symbiont recruitment and lignocellulose degradation in gut of black soldier fly larvae.Microbiome · 2026Article
- Bridging laboratory and field research: Method adjustments to blood feed field-derived Aedes aegypti.PLoS neglected tropical diseases · 2026Article
- Bacterially expressed recombinant TMOF induces mortality and gut microbial alterations in Aedes albopictus larvae.Scientific reports · 2026Article
- The microbiota impacts life history traits and mating success in malebioRxiv : the preprint server for biology · 2026Article
- Addition of a single bacterial isolate to conventional larval rearing water can impact wing size and longevity in adult malebioRxiv : the preprint server for biology · 2026Article
- Preoperative gut microbiota combined with machine learning for predicting post-hepatectomy liver failure in HBV-related hepatocellular carcinoma: a pilot study.Frontiers in cellular and infection microbiology · 2026Article
- Insect Lipid Metabolism in the Presence of Symbiotic and Pathogenic Viruses and Bacteria.Advances in experimental medicine and biology · 2026Review
- Transplant of cryopreserved mosquito gut microbiota reveals strain-specific patterns of microbial acquisition and assembly.ISME host microbe · 2026Article
- Mosquitoes as vector-based sentinels for tracking bacterial pathogens and antimicrobial resistance in the environment.Frontiers in microbiology · 2026Review
- Blood feeding, microbiota, and layered mosquito immunity: from pathway phenotypes to vector competence.Frontiers in immunology · 2026Review
- Article
- Evaluation ofiScience · 2025Article
- Identification of a culturable fungal species and endosymbiotic bacteria in saliva of Aedes aegypti and Culex pipiens and their impact on arbovirus infection in vitro.Parasites & vectors · 2025Article
- Historical mosquito colonization dynamics are associated with patterns of microbial community assembly in aboveground aquatic habitats.Environmental microbiome · 2025Article
- Selected gut bacteria promote mosquito larval growth through the regulation of ionic microenvironment and protease expression.iScience · 2025Article
- tRNA expression and modifications as critical components in the biology of blood-feeding arthropods.Current opinion in insect science · 2025Review
- Holobiont-holobiont interactions across host-ectoparasite systems.Parasites & vectors · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mosquito microbiota impacts the physiology of its host and is essential for normal larval development, thereby influencing transmission of vector-borne pathogens. Germ-free mosquitoes generated with current methods show larval stunting and developmental deficits. Therefore, functional studies of the mosquito microbiota have so far mostly been limited to antibiotic treatments of emerging adults. In this study, we introduce a method to produce germ-free Aedes aegypti mosquitoes. It is based on reversible colonisation with bacteria genetically modified to allow complete decolonisation at any developmental stage. We show that, unlike germ-free mosquitoes previously produced using sterile diets, reversibly colonised mosquitoes show no developmental retardation and reach the same size as control adults. This allows us to uncouple the study of the microbiota in larvae and adults. In adults, we detect no impact of bacterial colonisation on mosquito fecundity or longevity. In larvae, data from our transcriptome analysis and diet supplementation experiments following decolonisation suggest that bacteria support larval development by contributing to folate biosynthesis and by enhancing energy storage. Our study establishes a tool to study the microbiota in insects and deepens our knowledge on the metabolic contribution of bacteria to mosquito development.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.