Evidence mapPaperPMID 33595813Full record

ArticleGenes & genomics2021

Knockdown of eIF3a attenuated cell growth in K1 human thyroid cancer cells.

Xucai Zheng, Shengying Wang, Shikai Hong, Jianjun Liu, Chenghao Jiang

Abstract read
PubMed Publisher
In one paragraph

Article in Genes & genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Xucai ZhengDepartment of Head and Neck, Breast Surgery, the First Affiliated Hospital of University of Science and Technology of China, Anhui Provincial Cancer Hospital, No.107 Huanhu East Road, Hefei, 230001, China.
Shengying WangDepartment of Head and Neck, Breast Surgery, the First Affiliated Hospital of University of Science and Technology of China, Anhui Provincial Cancer Hospital, No.107 Huanhu East Road, Hefei, 230001, China. shengying0226@163.com.
Shikai HongDepartment of Head and Neck, Breast Surgery, the First Affiliated Hospital of University of Science and Technology of China, Anhui Provincial Cancer Hospital, No.107 Huanhu East Road, Hefei, 230001, China.
Jianjun LiuDepartment of Head and Neck, Breast Surgery, the First Affiliated Hospital of University of Science and Technology of China, Anhui Provincial Cancer Hospital, No.107 Huanhu East Road, Hefei, 230001, China.
Chenghao JiangDepartment of Head and Neck, Breast Surgery, the First Affiliated Hospital of University of Science and Technology of China, Anhui Provincial Cancer Hospital, No.107 Huanhu East Road, Hefei, 230001, China.
University of Science and Technology of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn ribosome establishment and the initiation of translation, eukaryotic translation initiation factor (eIF) 3a is a pivotal functional subunit of the eIF3 complex. In various cancer types, abnormal eIF3a expression plays an important role in tumorigenesis.

objectiveWe aimed to explore the role of eIF3a in human thyroid cancer (TC). MATERIAL AND

methodsThe expression of eIF3a was determined in TC tissues by qRT-PCR and immunohistochemistry (IHC) assay, respectively. In addition, the expression of eIF3a in K1 and BCPAP cells were detected by qRT-PCR. Cell proliferation, cell cycle, and cell apoptosis were assessed after eIF3a knockdown in K1 in cell line.

resultsThe expression of eIF3a mRNA was high in TC tissues and cancer cell lines. Moreover, eIF3a expression in TC tissues indicated that high eIF3a level was associated with tumor grade. In addition, eIF3a knockdown resulted in a significantly decrease in cell proliferation and increased the apoptosis of K1 cells. Cell cycle was arrested in both the S and G2/M phase. The levels of phosphorylated ERK1/2 and surviving were decreased after eIF3a knockdown.

conclusionOur study suggested that eIF3a contributed to TC cell proliferation. It may be a promising target for gene therapy in human thyroid cancer.

Indexed as

ApoptosisCell Cycle CheckpointsCell Line, TumorCell ProliferationCell SurvivalEukaryotic Initiation Factor-3FemaleGene Knockdown TechniquesHumansMaleMiddle AgedNeoplasm StagingThyroid NeoplasmsEIF3A protein, humanEukaryotic Initiation Factor-3ApoptosisERK1/2ProliferationSurvivinThyroid cancer

Identifiers

PMID33595813
OpenAlexW3131031307

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.