Evidence map›Paper›PMID 33602182›Full record

ArticleBMC complementary medicine and therapies2021

Porcine placenta extract improves high-glucose-induced angiogenesis impairment.

Chatchai Nensat, Worawat Songjang, Rutaiwan Tohtong, Tuangporn Suthiphongchai, Suchada Phimsen, Panthip Rattanasinganchan, Pornphimon Metheenukul, Sarawut Kumphune, Arunya Jiraviriyakul

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC complementary medicine and therapies, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.

  1. Pooled it
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  11. Perinatal derivatives: How to best characterize their multimodal functionsFrontiers in bioengineering and biotechnology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Chatchai NensatIntegrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand.
Worawat SongjangIntegrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand.
Rutaiwan TohtongDepartment of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Tuangporn SuthiphongchaiDepartment of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Suchada PhimsenDepartment of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
Panthip RattanasinganchanFaculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540, Thailand.
Pornphimon MetheenukulDepartment of Veterinary Technology, Faculty of Veterinery Technology, Kasetsart University, Bangkok, 10900, Thailand.
Sarawut KumphuneIntegrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand.
Arunya JiraviriyakulIntegrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand. arunyaj@nu.ac.th.ORCID http://orcid.org/0000-0003-0122-5984
Naresuan University · THMahidol University · THChiang Mai University · THHuachiew Chalermprakiet University · THKasetsart University · TH

Funding

Mahidol University B05F630066
6 · The paper itself

Abstract

backgroundHigh glucose (HG)-induced reactive oxygen species (ROS) overproduction impairs angiogenesis that is one pivotal factor of wound healing process. Angiogenesis impairment induces delayed wound healing, whereby it eventually leads to amputation in cases of poorly controlled diabetes with diabetic ulceration. Porcine placenta extract (PPE) is a natural waste product that comprises plenty of bioactive agents including growth factors and antioxidants. It was reported as an effective compound that prevents ROS generation. The goal of this study was to investigate the in vitro effect of PPE on HG-induced ROS-mediated angiogenesis impairment.

methodsPrimary endothelial cells (HUVECs) and endothelial cell line (EA.hy926) were treated with HG in the presence of PPE. The endothelial cells (ECs) viability, intracellular ROS generation, migration, and angiogenesis were determined by MTT assay, DCFDA reagent, wound healing assay, and tube formation assay, respectively. Additionally, the molecular mechanism of PPE on HG-induced angiogenesis impairment was investigated by Western blot. The angiogenic growth factor secretion was also investigated by the sandwich ELISA technique.

resultsHG in the presence of PPE significantly decreased intracellular ROS overproduction compared to HG alone. HG in the presence of PPE significantly increased ECs viability, migration, and angiogenesis compared to HG alone by showing recovery of PI3K/Akt/ERK1/2 activation. HG in the presence of PPE also decreased ECs apoptosis compared to HG alone by decreasing p53/Bax/cleaved caspase 9/cleaved caspase 3 levels and increasing Bcl 2 level.

conclusionPPE attenuated HG-induced intracellular ROS overproduction that improved ECs viability, proliferation, migration, and angiogenesis by showing recovery of PI3K/Akt/ERK1/2 activation and inhibition of ECs apoptosis. This study suggests PPE ameliorated HG-induced ROS-mediated angiogenesis impairment, whereby it potentially provides an alternative treatment for diabetic wounds.

Indexed as

SwineAnimalsAntioxidantsBiological ProductsCell LineCell MovementCell SurvivalDiabetes ComplicationsEndothelial CellsFemaleGlucoseHumansHuman Umbilical Vein Endothelial CellsIntercellular Signaling Peptides and ProteinsNeovascularization, PhysiologicOxidative StressAntioxidantsBCL2 protein, humanBiological ProductsGlucoseIntercellular Signaling Peptides and ProteinsPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-bcl-2Reactive Oxygen SpeciesAngiogenesis impairmentDiabetesEndothelial cellPorcine placenta extractWound healing

Identifiers

PMID33602182
PMCPMC7893890
OpenAlexW3130820346

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.