Evidence mapPaperPMID 33629656Full record

ArticleeLife2021

The SWELL1-LRRC8 complex regulates endothelial AKT-eNOS signaling and vascular function.

Ahmad F Alghanem, Javier Abello, Joshua M Maurer, Ashutosh Kumar, Chau My Ta, Susheel K Gunasekar, Urooj Fatima, Chen Kang, Litao Xie, Oluwaseun Adeola and 7 more

Open access · goldAbstract read
In one paragraph

Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 72 citations in OpenAlex.

  1. Article
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  4. Mechanosensitive ion channels as novel targets in osteoporosis.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 3 institutions in 2 countries.

Ahmad F AlghanemDepartment of Internal Medicine, Cardiovascular Division, Washington University School of Medicine, St. Louis, United States.ORCID 0000-0002-5823-3806
Javier AbelloDepartment of Cell Biology and Physiology, Washington University in St. Louis, School of Medicine, St. Louis, United States.
Joshua M MaurerDepartment of Internal Medicine, Cardiovascular Division, Washington University School of Medicine, St. Louis, United States.ORCID 0000-0002-2107-0767
Ashutosh KumarDepartment of Internal Medicine, Cardiovascular Division, Washington University School of Medicine, St. Louis, United States.
Chau My TaDepartment of Internal Medicine, Cardiovascular Division, Washington University School of Medicine, St. Louis, United States.
Susheel K GunasekarDepartment of Internal Medicine, Cardiovascular Division, Washington University School of Medicine, St. Louis, United States.
Urooj FatimaDepartment of Internal Medicine, Cardiovascular Division, University of Iowa, Iowa City, United States.
Chen KangDepartment of Internal Medicine, Cardiovascular Division, Washington University School of Medicine, St. Louis, United States.
Litao XieDepartment of Internal Medicine, Cardiovascular Division, Washington University School of Medicine, St. Louis, United States.
Oluwaseun AdeolaDepartment of Internal Medicine, Cardiovascular Division, University of Iowa, Iowa City, United States.
Megan RikerDepartment of Ophthalmology, University of Iowa, Carver College of Medicine, Iowa City, United States.
Macaulay Elliot-HudsonDepartment of Internal Medicine, Cardiovascular Division, University of Iowa, Iowa City, United States.
Rachel A MinerathDepartment of Internal Medicine, Cardiovascular Division, University of Iowa, Iowa City, United States.
Chad E GrueterDepartment of Internal Medicine, Cardiovascular Division, University of Iowa, Iowa City, United States.
Robert F MullinsDepartment of Ophthalmology, University of Iowa, Carver College of Medicine, Iowa City, United States.
Amber N StratmanDepartment of Cell Biology and Physiology, Washington University in St. Louis, School of Medicine, St. Louis, United States.ORCID 0000-0002-8111-4186
Rajan SahDepartment of Internal Medicine, Cardiovascular Division, Washington University School of Medicine, St. Louis, United States.ORCID 0000-0003-1092-1244
Washington University in St. Louis · USUniversity of Iowa · USKing Saud bin Abdulaziz University for Health Sciences · SA

Funding

Washington University Nutrition Obesity Research CenterP30DK056341 · WASHINGTON UNIVERSITY · 1999 to 2025
$5.7M
Pulmonary Development and Disease Pathogenesis Training ProgramT32HL007752 · CINCINNATI CHILDRENS HOSP MED CTR · 1994 to 2025
$1.6M
Analysis of Mural Cell Ontogeny and Gene Function During Vascular DevelopmentR00HL125683 · NHLBI · WASHINGTON UNIVERSITY · PI Amber Nicole Stratman · 2021 to 2021
$249k
BLRD VA I01 BX005072NHLBI NIH HHS R00 HL125683NHLBI NIH HHS R01 HL125436NHLBI NIH HHS T32 HL007752NIDDK NIH HHS P30 DK056341NIDDK NIH HHS R01 DK106009NIGMS NIH HHS R35 GM137976
6 · The paper itself

Abstract

The endothelium responds to numerous chemical and mechanical factors in regulating vascular tone, blood pressure, and blood flow. The endothelial volume-regulated anion channel (VRAC) has been proposed to be mechanosensitive and thereby sense fluid flow and hydrostatic pressure to regulate vascular function. Here, we show that the leucine-rich repeat-containing protein 8a, LRRC8A (SWELL1), is required for VRAC in human umbilical vein endothelial cells (HUVECs). Endothelial LRRC8A regulates AKT-endothelial nitric oxide synthase (eNOS) signaling under basal, stretch, and shear-flow stimulation, forms a GRB2-Cav1-eNOS signaling complex, and is required for endothelial cell alignment to laminar shear flow. Endothelium-restricted

Indexed as

AnimalsEndotheliumFemaleMaleMembrane ProteinsMiceNitric Oxide Synthase Type IIIProto-Oncogene Proteins c-aktSignal TransductionLRRC8A protein, mouseMembrane ProteinsNitric Oxide Synthase Type IIINos3 protein, mouseProto-Oncogene Proteins c-aktcell biologydiabeteshypertensionion channelmechanobiologymouse

Identifiers

PMID33629656
PMCPMC7997661
OpenAlexW3133311184

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.