ArticlePlant & cell physiology2021
ClearSeeAlpha: Advanced Optical Clearing for Whole-Plant Imaging.
Article in Plant & cell physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Tissue-specific recombination-activated clonal and inducible transcription (TRAC-IT) enables controlled gene activation in stably marked tissues.The Plant journal : for cell and molecular biology · 2026Article
- PAX-regulated self-organizing polarity drives procambial strand formation.Nature plants · 2026Article
- Rational design of T-DNA vectors enables predictable, single-copy integration inbioRxiv : the preprint server for biology · 2026Article
- Fluorescence lifetime-based multiplex imaging in living plant cells.Plant physiology · 2026Article
- Mutational analyses of an instability domain reveal its conserved role in the regulation of class-B ARF levels inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Temporal changes in surface tension guide microtubule organization and accurate asymmetric division of Arabidopsis zygotes.Nature communications · 2026Article
- Quantification of cell-type-specific plasmodesmata distribution in Arabidopsis roots reveals spatial and patterning dynamics.The Plant journal : for cell and molecular biology · 2026Article
- A Simple and Fast Optical Clearing Method for Whole-Mount Fluorescence In Situ Hybridization (FISH) Imaging.Journal of biophotonics · 2025Article
- MONOPTEROS isoform MP11ir plays a role during somatic embryogenesis in Arabidopsis thaliana.Plant physiology · 2025Article
- A simple and versatile plasma membrane staining method for visualizing living cell morphology in reproductive tissues across diverse plant species.Plant methods · 2025Article
- Neofunctionalized RGF pathways drive haustorial organogenesis in parasitic plants.Science advances · 2025Article
- Cartwheel aerenchyma iniScience · 2025Article
- Mago nashi controls auxin-mediated embryo patterning in Arabidopsis by regulating transcript abundance.The New phytologist · 2025Article
- A transcriptomic perspective of P trade in mycorrhizal grapevine.Mycorrhiza · 2025Article
- Dual and spatially resolved drought responses in the Arabidopsis leaf mesophyll revealed by single-cell transcriptomics.The New phytologist · 2025Article
- The protein kinases KIPK and KIPK-LIKE1 suppress overbending during negative hypocotyl gravitropic growth in Arabidopsis.The Plant cell · 2025Article
- The PEBP genes FLOWERING LOCUS T and TERMINAL FLOWER 1 modulate seed dormancy and size.Journal of experimental botany · 2025Article
- Analysis of auxin responses in the fern Ceratopteris richardii identifies the developmental phase as a major determinant for response properties.Development (Cambridge, England) · 2024Article
- Reproducibly oriented cell divisions pattern the prothallus to set up dorsoventrality and de novo meristem formation in Marchantia polymorpha.Current biology : CB · 2024Article
- The Chaperone NASP Contributes to de Novo Deposition of the Centromeric Histone Variant CENH3 in Arabidopsis Early Embryogenesis.Plant & cell physiology · 2024Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
To understand how the body of plants is made, it is essential to observe the morphology, structure and arrangement of constituent cells. However, the opaque nature of the plant body makes it difficult to observe the internal structures directly under a microscope. To overcome this problem, we developed a reagent, ClearSee, that makes plants transparent, allowing direct observation of the inside of a plant body without inflicting damage on it, e.g. through physical cutting. However, because ClearSee is not effective in making some plant species and tissues transparent, in this study, we further improved its composition to prevent oxidation, and have developed ClearSeeAlpha, which can be applied to a broader range of plant species and tissues. Sodium sulfite, one of the reductants, prevented brown pigmentation due to oxidation during clearing treatment. Using ClearSeeAlpha, we show that it is possible to obtain clear chrysanthemum leaves, tobacco and Torenia pistils and fertilized Arabidopsis thaliana fruits-tissues that have hitherto been challenging to clear. Moreover, we show that the fluorescence intensity of purified fluorescent proteins emitting light of various colors was unaffected in the ClearSeeAlpha solution; only the fluorescence intensity of TagRFP was reduced by about half. ClearSeeAlpha should be useful in the discovery and analysis of biological phenomena occurring deep inside the plant tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.