Evidence map›Paper›PMID 33640452›Full record

ArticleThe Journal of biological chemistry

Ectopically expressed olfactory receptors OR51E1 and OR51E2 suppress proliferation and promote cell death in a prostate cancer cell line.

Alexey Pronin, Vladlen Slepak

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed, 1 pooled it
3.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.

  1. Microbial volatile organic compounds and olfactory receptors in wound malodor.Frontiers in cellular and infection microbiology · 2026
    Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Alexey ProninDepartment of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, Florida, USA. Electronic address: a.pronin@med.miami.edu.
Vladlen SlepakDepartment of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, Florida, USA. Electronic address: vslepak@med.miami.edu.
University of Miami · US

Funding

A robust assay for detecting agonist bias in living cells.R44GM125390 · NIGMS · MONTANA MOLECULAR, LLC · PI HUGHES, THOMAS E, QUINN, ANNE MARIE · 2019 to 2020
$1.2M
Neuronal control of body weight and size by the Gbeta5-R7 signaling complexR56DK119262 · NIDDK · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI SLEPAK, VLADLEN Z · 2019 to 2019
$192k
NIDDK NIH HHS R56 DK119262NIGMS NIH HHS R44 GM125390
6 · The paper itself

Abstract

Olfactory receptors (ORs), the largest family of G protein-coupled receptors, are expressed in the nasal epithelium where they mediate the sense of smell. However, ORs are also found in other non-nasal tissues, but the role of these ectopic ORs in cell signaling, proliferation, and survival is not well understood. Here, using an inducible expression system in the lymph node carcinoma of the prostate (LNCaP) cell line, we investigated two ectopic ORs, OR51E1 and OR51E2, which have been shown to be upregulated in prostate cancer. We found that, consistent with previous studies, OR51E1 stimulated adenylyl cyclase in response to treatment by short-chain to medium-chain organic acids (C3-C9) but not by acetate. OR51E2 responded to acetate and propionate but not to the longer chain organic acids. Stimulation of LNCaP cells with butyrate inhibited their growth, and the knockdown of the endogenous OR51E1 negated this cytostatic effect. Most significantly, overexpression of OR51E1 or OR51E2 suppressed LNCaP cell proliferation. Overexpression of another ectopic OR OR2AT4, β2-adrenergic receptor, or treatment of cells with forskolin did not suppress cell proliferation, indicating that a rise in cAMP is not sufficient to induce cytostasis. Overexpression of OR51E1 caused an upregulation of cytostatic and cell death markers including p27, p21, and p53, strongly increased annexin V staining, and stimulated extracellular signal-regulated protein kinases 1 and 2. Overexpression and/or activation of OR51E1 did not affect human embryonic kidney 293 cell proliferation, indicating that cytotoxicity of OR51E1/OR51E2 is specific for LNCaP cells. Together, our results further our understanding of prostate cancer etiology and suggest that ectopic ORs may be useful therapeutic targets.

Indexed as

Cell DeathCell Line, TumorCell ProliferationEctopic Gene ExpressionHEK293 CellsHumansMaleNeoplasm ProteinsProstateProstatic NeoplasmsReceptors, G-Protein-CoupledReceptors, OdorantSignal TransductionNeoplasm ProteinsOR2AT4 protein, humanOR51E1 protein, humanOR51E2 protein, humanReceptors, G-Protein-CoupledReceptors, OdorantcAMPcell deathG proteinG protein–coupled receptorolfactoryOR51E1OR51E2proliferation

Identifiers

PMID33640452
PMCPMC8024707
OpenAlexW3130624037

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.