ArticleLife science alliance2021
nNOS regulates ciliated cell polarity, ciliary beat frequency, and directional flow in mouse trachea.
Article in Life science alliance, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Novel mechanisms of chemosensory adaptation to the cave environment.bioRxiv : the preprint server for biology · 2026Article
- Measurement of nitric oxide production in mouse tracheal epithelial cell cultures differentiated at the air liquid interface.bioRxiv : the preprint server for biology · 2025Article
- Moderate levels of folic acid benefit outcomes for cilia based neural tube defects.Developmental biology · 2025Article
- Structure and function relationships of mucociliary clearance in human and rat airways.Nature communications · 2025Article
- Structure and Function Relationships of Mucociliary Clearance in Human and Rat Airways.bioRxiv : the preprint server for biology · 2025Article
- Genomic attributes of airway commensal bacteria and mucosa.Communications biology · 2024Article
- Impaired Nitric Oxide Synthetase Activity in Primary Ciliary Dyskinesia-Data-Driven Hypothesis.Journal of clinical medicine · 2023Article
- Quantifying cilia beat frequency using high-speed video microscopy: Assessing frame rate requirements when imaging different ciliated tissues.Physiological reports · 2022Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Clearance of the airway is dependent on directional mucus flow across the mucociliary epithelium, and deficient flow is implicated in a range of human disorders. Efficient flow relies on proper polarization of the multiciliated cells and sufficient ciliary beat frequency. We show that NO, produced by nNOS in the multiciliated cells of the mouse trachea, controls both the planar polarity and the ciliary beat frequency and is thereby necessary for the generation of the robust flow. The effect of nNOS on the polarity of ciliated cells relies on its interactions with the apical networks of actin and microtubules and involves RhoA activation. The action of nNOS on the beat frequency is mediated by guanylate cyclase; both NO donors and cGMP can augment fluid flow in the trachea and rescue the deficient flow in nNOS mutants. Our results link insufficient availability of NO in ciliated cells to defects in flow and ciliary activity and may thereby explain the low levels of exhaled NO in ciliopathies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.