Evidence mapPaperPMID 33658008Full record

ArticleBMC cardiovascular disorders2021

microRNA-130a-5p suppresses myocardial ischemia reperfusion injury by downregulating the HMGB2/NF-κB axis.

Yong Li, Hongbo Zhang, Zhanhu Li, Xiaoju Yan, Yuan Li, Shuai Liu

Open access · goldAbstract read
In one paragraph

Article in BMC cardiovascular disorders, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.

  1. Noncoding RNA as potential therapeutics to rescue mitochondrial dysfunction in cardiovascular diseases.American journal of physiology. Heart and circulatory physiology · 2025
    Pooled it
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Bioengineered · 2022
    Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Yong LiDepartment of Cardiology, Harrision International Peace Hospital, No. 180 Renmin East Road, Hengshui, 053000, Hebei, People's Republic of China. drliyong0927@163.com.ORCID 0000-0003-0889-9439
Hongbo ZhangDepartment of Cardiology, Harrision International Peace Hospital, No. 180 Renmin East Road, Hengshui, 053000, Hebei, People's Republic of China.
Zhanhu LiDepartment of Cardiology, Harrision International Peace Hospital, No. 180 Renmin East Road, Hengshui, 053000, Hebei, People's Republic of China.
Xiaoju YanDepartment of Cardiology, Harrision International Peace Hospital, No. 180 Renmin East Road, Hengshui, 053000, Hebei, People's Republic of China.
Yuan LiDepartment of Cardiology, Harrision International Peace Hospital, No. 180 Renmin East Road, Hengshui, 053000, Hebei, People's Republic of China.
Shuai LiuDepartment of Cardiology, Harrision International Peace Hospital, No. 180 Renmin East Road, Hengshui, 053000, Hebei, People's Republic of China.
Harrison International Peace Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMyocardial ischemia reperfusion injury (MIRI) is defined as tissue injury in the pathological process of progressive aggravation in ischemic myocardium after the occurrence of acute coronary artery occlusion. Research has documented the involvement of microRNAs (miRs) in MIRI. However, there is obscure information about the role of miR-130a-5p in MIRI. Herein, this study aims to investigate the effect of miR-130a-5p on MIRI.

methodsMIRI mouse models were established. Then, the cardiac function and hemodynamics were detected using ultrasonography and multiconductive physiological recorder. Functional assays in miR-130a-5p were adopted to test the degrees of oxidative stress, mitochondrial functions, inflammation and apoptosis. Hematoxylin and eosin (HE) staining was performed to validate the myocardial injury in mice. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was employed to assess the expression patterns of miR-130a-5p, high mobility group box (HMGB)2 and NF-κB. Then, dual-luciferase reporter gene assay was performed to elucidate the targeting relation between miR-130a-5p and HMGB2.

resultsDisrupted structural arrangement in MIRI mouse models was evident from HE staining. RT-qPCR revealed that overexpressed miR-130a-5p alleviated MIRI, MIRI-induced oxidative stress and mitochondrial disorder in the mice. Next, the targeting relation between miR-130a-5p and HMGB2 was ascertained. Overexpressed HMGB2 annulled the protective effects of miR-130a-5p in MIRI mice. Additionally, miR-130a-5p targets HMGB2 to downregulate the nuclear factor kappa-B (NF-κB) axis, mitigating the inflammatory injury induced by MIRI.

conclusionOur study demonstrated that miR-130a-5p suppresses MIRI by down-regulating the HMGB2/NF-κB axis. This investigation may provide novel insights for development of MIRI treatments.

Indexed as

AnimalsDisease Models, AnimalDown-RegulationHMGB2 ProteinMaleMiceMice, Inbred C57BLMicroRNAsMitochondria, HeartMyocardial Reperfusion InjuryMyocardiumNF-kappa BOxidative StressPhosphorylationSignal TransductionHMGB2 ProteinMicroRNAsMIRN130 microRNA, mouseNF-kappa BHMGB2microRNA-130a-5pMitochondrial disorderMyocardium ischemia reperfusion injuryNF-κb pathwayOxidative stress

Identifiers

PMID33658008
PMCPMC7931544
OpenAlexW3133583687

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.