Evidence map›Paper›PMID 33660772›Full record

ArticleNucleic acids research2021

A RanBP2-type zinc finger protein functions in intron splicing in Arabidopsis mitochondria and is involved in the biogenesis of respiratory complex I.

Stéphane Bentolila, Andrew B Gipson, Alexander J Kehl, Lauren N Hamm, Michael L Hayes, R Michael Mulligan, Maureen R Hanson

Open access · goldAbstract read
In one paragraph

Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Review
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  6. Review
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  9. Plant organellar RNA maturation.The Plant cell · 2023
    Review
  10. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Stéphane BentolilaDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Andrew B GipsonDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Alexander J KehlDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Lauren N HammDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Michael L HayesDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA.
R Michael MulliganDepartment of Developmental and Cell Biology, University of California Irvine, Irvine, CA 90032, USA.
Maureen R HansonDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Cornell University · USCalifornia State University Los Angeles · USUniversity of California, Irvine · US

Funding

Zeiss LSM710 Confocal Microscope for Shared Imaging FacilityS10RR025502 · NCRR · CORNELL UNIVERSITY · PI WILLIAMS, REBECCA M · 2009 to 2009
$500k
NCRR NIH HHS S10 RR025502
6 · The paper itself

Abstract

The RanBP2 zinc finger (Znf) domain is a prevalent domain that mediates protein interaction and RNA binding. In Arabidopsis, a clade of four RanBP2 Znf-containing proteins, named the Organelle Zinc (OZ) finger family, are known or predicted to be targeted to either the mitochondria or the plastids. Previously we reported that OZ1 is absolutely required for the editing of 14 sites in chloroplasts. We now have investigated the function of OZ2, whose null mutation is embryo lethal. We rescued the null mutant by expressing wild-type OZ2 under the control of the seed-specific ABSCISIC ACID-INSENSITIVE3 (ABI3) promoter. Rescued mutant plants exhibit severely delayed development and a distinctive morphological phenotype. Genetic and biochemical analyses demonstrated that OZ2 promotes the splicing of transcripts of several mitochondrial nad genes and rps3. The splicing defect of nad transcripts results in the destabilization of complex I, which in turn affects the respiratory ability of oz2 mutants, turning on the alternative respiratory pathway, and impacting the plant development. Protein-protein interaction assays demonstrated binding of OZ2 to several known mitochondrial splicing factors targeting the same splicing events. These findings extend the known functional repertoire of the RanBP2 zinc finger domain in nuclear splicing to include plant organelle splicing.

Indexed as

RNA SplicingArabidopsisArabidopsis ProteinsElectron Transport Complex IGenes, LethalIntronsMitochondriaMitochondrial ProteinsMutationPromoter Regions, GeneticRibosomal ProteinsRNA Splicing FactorsZincArabidopsis ProteinsElectron Transport Complex IMitochondrial ProteinsRibosomal ProteinsRNA Splicing FactorsRPS3 protein, ArabidopsisZinc

Identifiers

PMID33660772
PMCPMC8034646
OpenAlexW3135869923

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.