Evidence map›Paper›PMID 33664283›Full record

ArticleScientific reports2021

Distinct effects of V617F and exon12-mutated JAK2 expressions on erythropoiesis in a human induced pluripotent stem cell (iPSC)-based model.

Nungruthai Nilsri, Panchalee Jangprasert, Jaturawat Pawinwongchai, Nipan Israsena, Ponlapat Rojnuckarin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. CRISPR/Cas9-Based Modeling ofInternational journal of molecular sciences · 2025
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  4. Review
  5. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Nungruthai NilsriDoctor of Philosophy Program in Medical Sciences, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Panchalee JangprasertInterdisciplinary Program of Biomedical Sciences, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Jaturawat PawinwongchaiFaculty of Medical Technology, Rangsit University, Pathum Thani, Thailand.
Nipan IsrasenaStem Cell and Cell Therapy Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Ponlapat RojnuckarinResearch Unit in Translational Hematology, Division of Hematology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Bangkok, 10330, Thailand. Ponlapat.R@Chula.ac.th.
Chulalongkorn University · THKing Chulalongkorn Memorial Hospital · THRangsit University · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Activating mutations affecting the JAK-STAT signal transduction is the genetic driver of myeloproliferative neoplasms (MPNs) which comprise polycythemia vera (PV), essential thrombocythemia (ET) and myelofibrosis. The JAK2p.V617F mutation can produce both erythrocytosis in PV and thrombocytosis in ET, while JAK2 exon 12 mutations cause only erythrocytosis. We hypothesized that these two mutations activated different intracellular signals. In this study, the induced pluripotent stem cells (iPSCs) were used to model JAK2-mutated MPNs. Normal iPSCs underwent lentiviral transduction to overexpress JAK2p.V617F or JAK2p.N542_E543del (JAK2exon12) under a doxycycline-inducible system. The modified iPSCs were differentiated into erythroid cells. Compared with JAK2V617F-iPSCs, JAK2exon12-iPSCs yielded more total CD71

Indexed as

ErythropoiesisExonsGene Expression RegulationHumansInduced Pluripotent Stem CellsJanus Kinase 2MutationMyeloproliferative DisordersPolycythemiaPolycythemia VeraPrimary MyelofibrosisSTAT1 Transcription FactorSTAT3 Transcription FactorSTAT Transcription FactorsThrombocythemia, EssentialThrombocytosisJAK2 protein, humanJanus Kinase 2STAT1 Transcription FactorSTAT3 Transcription FactorSTAT Transcription Factors

Identifiers

PMID33664283
PMCPMC7933160
OpenAlexW3135028563

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.