ArticleThe New phytologist2021
Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA-induced gene knockouts.
Article in The New phytologist, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Wood Formation Modeling - A Research Review and Future Perspectives.Frontiers in plant science · 2022Pooled it
- Overexpression ofPlants (Basel, Switzerland) · 2026Article
- Article
- Revealing the genetic regulation of wood traits and secondary cell wall development inHorticulture research · 2026Article
- Article
- Genome-wide analysis of the CesA gene family in Brassica napus under abiotic stress and functional study of in root growth.BMC plant biology · 2026Article
- Article
- Overexpression of a Xylem-Dominant Expressing BTB Gene,Plants (Basel, Switzerland) · 2025Article
- Genetic manipulation of a COBRA gene, PtrCOB11, substantially alters wood properties in poplar.Plant biotechnology journal · 2025Article
- PtrVINV2 is dispensable for cellulose synthesis but essential for salt tolerance in Populus trichocarpa Torr. and Gray.Plant biotechnology journal · 2025Article
- Generation and Functional Characteristics of CRISPR/Cas9-EditedPlants (Basel, Switzerland) · 2025Article
- Genome-wide analysis of the cellulose synthase gene family in autopolyploid sugarcane (Frontiers in plant science · 2025Article
- Simultaneous regulation of both lignin and cellulose biosynthesis modifies xylem fiber properties inFrontiers in plant science · 2025Article
- The transcriptional regulation of a putative hemicellulose gene, PtrPARVUS2 in poplar.Scientific reports · 2024Article
- Comparative proteomic analysis provides insights into wood formation in immature xylem at different ages inFrontiers in plant science · 2024Article
- Meliaceae genomes provide insights into wood development and limonoids biosynthesis.Plant biotechnology journal · 2023Article
- Cas9/gRNA-Mediated Mutations inInternational journal of molecular sciences · 2022Article
- Aspartic proteases modulate programmed cell death and secondary cell wall synthesis during wood formation in poplar.Journal of experimental botany · 2022Article
- Chromosome-scale genome assembly provides insights into the molecular mechanisms of tissue development of Populus wilsonii.Communications biology · 2022Article
- Molecular studies of cellulose synthase supercomplex from cotton fiber reveal its unique biochemical properties.Science China. Life sciences · 2022Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant cellulose is synthesized by a large plasma membrane-localized cellulose synthase (CesA) complex. However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts. Here, the Cas9/gRNA-induced knockouts of PtrCesA4, 7A, 7B, 8A and 8B genes were produced in Populus trichocarpa. Based on anatomical, immunohistochemical and wood composition evidence, we gained a comprehensive understanding of five SCW PtrCesAs at the genetic level. Complete loss of PtrCesA4, 7A/B or 8A/B led to similar morphological abnormalities, indicating similar and nonredundant genetic functions. The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre. In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary). Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures. Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
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