SynthesisPloS one2021
Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review.
Synthesis in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Measles Vaccine Coverage and Disease Outbreaks: A Systematic Review of the Early Impact of COVID-19 in Low and Lower-Middle Income Countries.International journal of public health · 2024Pooled it
- Comparison of Cobas Plasma Separation Cards and Whatman filter paper cards versus conventional serum samples for anti-Trypansoma cruzi antibody detection.PLoS neglected tropical diseases · 2026Article
- Comparison of capillary microsampling and venous blood for multi-pathogen serosurveillance.Scientific reports · 2026Article
- Evaluation of the diagnostic performance of a commercial ELISA based on two recombinant antigens for the diagnosis of Strongyloides stercoralis infection.Parasites & vectors · 2026Article
- Sources and transmission dynamics ofmedRxiv : the preprint server for health sciences · 2026Article
- Schistosomiasis in Western Lake Turkana, Kenya: An Exploratory Serosurvey and Validation of Dried Blood Spots for Field Studies.Tropical medicine and infectious disease · 2026Article
- Using dried blood spots to estimate Toxoplasma gondii seroprevalence in pregnant women in Catalonia, Spain, and to serologically diagnose congenital toxoplasmosis.PLoS neglected tropical diseases · 2026Article
- Decoupling of maternal and neonatal inflammatory levels at the maternal-fetal interface: evidence from a population-based proteomic study.Frontiers in immunology · 2026Article
- Community-Engaged Approach to Increase Physical Activity Among Black Individuals With Colorectal Cancer: Protocol for a Feasibility Randomized Controlled Trial of the Physical Activity Centers Empowerment Study.JMIR research protocols · 2025Article
- Discovery of molecular signature of long-term psychiatric sequelae in COVID-19 through proteome profiling of dried blood spots.Translational psychiatry · 2025Article
- Children with hepatitis B virus infections, Democratic Republic of the Congo.Bulletin of the World Health Organization · 2025Article
- Dried Spot Paradigm: Problems and Prospects in Proteomics.International journal of molecular sciences · 2025Review
- Applying novel Plasmodium Vivax serological exposure markers to quantify residual malaria transmission in the Philippines through repeated health facility surveys: the SMaRT study protocol.BMC infectious diseases · 2025Article
- High correlation between detection of dengue IgG from dried blood spots and serum using an indirect IgG ELISA assay: A validation study in Fortaleza, Brazil.PLoS neglected tropical diseases · 2025Article
- Integrated Serosurveillance of Infectious Diseases Using Multiplex Bead Assays: A Systematic Review.Tropical medicine and infectious disease · 2025Review
- Article
- Optimization of dried blood spot for hepatitis B virus surface antibody quantification.PloS one · 2025Article
- Influence of Hematocrit and Storage Temperature on the Stability of Dried Blood Samples in Serological Analyses of Tetanus, Diphtheria, and Pertussis.Diagnostics (Basel, Switzerland) · 2024Article
- RSV Neutralizing Antibodies in Dried Blood.The Journal of infectious diseases · 2024Article
- Characterizing hepatitis B virus infection in children in the Democratic Republic of Congo to inform elimination efforts.medRxiv : the preprint server for health sciences · 2024Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundIncreasingly, vaccine efficacy studies are being recommended in low-and-middle-income countries (LMIC), yet often facilities are unavailable to take and store infant blood samples correctly. Dried blood spots (DBS), are useful for collecting blood from infants for diagnostic purposes, especially in low-income settings, as the amount of blood required is miniscule and no refrigeration is required. Little is known about their utility for antibody studies in children. This systematic review aims to investigate the correlation of antibody concentrations against infectious diseases in DBS in comparison to serum or plasma samples that might inform their use in vaccine clinical trials. METHODS AND
findingsWe searched MEDLINE, Embase and the Cochrane library for relevant studies between January 1990 to October 2020 with no language restriction, using PRISMA guidelines, investigating the correlation between antibody concentrations in DBS and serum or plasma samples, and the effect of storage temperature on DBS diagnostic performance. We included 40 studies in this systematic review. The antibody concentration in DBS and serum/plasma samples reported a good pooled correlation, (r2 = 0.86 (ranged 0.43 to 1.00)). Ten studies described a decline of antibody after 28 days at room temperature compared to optimal storage at -20°C, where antibodies were stable for up to 200 days. There were only five studies of anti-bacterial antibodies.
conclusionsThere is a good correlation between antibody concentrations in DBS and serum/plasma samples, supporting the wider use of DBS in vaccine and sero-epidemiological studies, but there is limited data on anti-bacterial antibodies. The correct storage of DBS is critical and may be a consideration for longer term storage.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.