Evidence map›Paper›PMID 33744339›Full record

ArticleNeuropharmacology2021

cAMP signaling through protein kinase A and Epac2 induces substance P release in the rat spinal cord.

Wenling Chen, James A McRoberts, Helena S Ennes, Juan Carlos Marvizon

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuropharmacology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Wenling ChenVatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, 90095, USA; Veteran Affairs Greater Los Angeles Healthcare System, Los Angeles, CA, 90073, USA.
James A McRobertsVatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, 90095, USA.
Helena S EnnesVatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, 90095, USA.
Juan Carlos MarvizonVatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, 90095, USA; Veteran Affairs Greater Los Angeles Healthcare System, Los Angeles, CA, 90073, USA. Electronic address: marvizon@g.ucla.edu.
University of California, Los Angeles · USVA Greater Los Angeles Healthcare System · US

Funding

NMDA Receptors in Primary AfferentsR01DA033059 · NIDA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MARVIZON, JUAN CARLOS GARCIA, MCROBERTS, JAMES A · 2012 to 2016
$1.4M
Neurotransmitter control of substance P release in the spinal cordI01RX000378 · VA · VA GREATER LOS ANGELES HEALTHCARE SYSTEM · PI MARVIZON, JUAN CARLOS GARCIA · 2012 to 2014
–
Latent Sensitization and Neuropathic PainI01RX001646 · VA · VA GREATER LOS ANGELES HEALTHCARE SYSTEM · PI MARVIZON, JUAN CARLOS GARCIA · 2016 to 2018
–
NIDA NIH HHS R01 DA033059RRD VA I01 RX000378RRD VA I01 RX001646
6 · The paper itself

Abstract

Using neurokinin 1 receptor (NK1R) internalization to measure of substance P release in rat spinal cord slices, we found that it was induced by the adenylyl cyclase (AC) activator forskolin, by the protein kinase A (PKA) activators 6-Bnz-cAMP and 8-Br-cAMP, and by the activator of exchange protein activated by cAMP (Epac) 8-pCPT-2-O-Me-cAMP (CPTOMe-cAMP). Conversely, AC and PKA inhibitors decreased substance P release induced by electrical stimulation of the dorsal root. Therefore, the cAMP signaling pathway mediates substance P release in the dorsal horn. The effects of forskolin and 6-Bnz-cAMP were not additive with NMDA-induced substance P release and were decreased by the NMDA receptor blocker MK-801. In cultured dorsal horn neurons, forskolin increased NMDA-induced Ca

Indexed as

AnimalsCells, CulturedColforsinCyclic AMPCyclic AMP-Dependent Protein KinasesGuanine Nucleotide Exchange FactorsHyperalgesiaMaleOrgan Culture TechniquesPosterior Horn CellsRatsRats, Sprague-DawleySignal TransductionSpinal CordSubstance P8-(4-chlorophenylthio)-2'-O-methyladenosine-3',5'-monophosphate acetoxymethyl esterColforsinCyclic AMPCyclic AMP-Dependent Protein KinasesGuanine Nucleotide Exchange FactorsRapgef4 protein, ratSubstance PCyclic-AMPHyperalgesiaNeurokinin-1 receptorNMDA receptorPrimary afferentProtein kinase ASubstance PVoltage-gated calcium channel

Identifiers

PMID33744339
PMCPMC8577816
OpenAlexW3138267792

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.