Evidence mapPaperPMID 33748285Full record

ArticleJournal of diabetes research2021

Inhibition of miRNA-155 Alleviates High Glucose-Induced Podocyte Inflammation by Targeting SIRT1 in Diabetic Mice.

Xiaolei Wang, Yanbin Gao, Wenming Yi, Yu Qiao, Hao Hu, Ying Wang, Yan Hu, Shuxin Wu, Hongfeng Sun, Taojing Zhang

Open access · goldAbstract read
In one paragraph

Article in Journal of diabetes research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 24 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Xiaolei WangDepartment of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.
Yanbin GaoSchool of Traditional Chinese Medicine, Capital Medical University, 10 Youanmenwai, Xitoutiao, Fengtai District, Beijing, China.
Wenming YiDepartment of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.
Yu QiaoDepartment of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.ORCID https://orcid.org/0000-0003-4670-724X
Hao HuDepartment of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.
Ying WangDepartment of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.
Yan HuDepartment of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.
Shuxin WuDepartment of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.
Hongfeng SunDepartment of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.
Taojing ZhangDepartment of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.ORCID https://orcid.org/0000-0002-4468-0664
Beijing University of Chinese Medicine · CNCapital Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveMicroinflammation plays a crucial role in podocyte dysfunction in diabetic nephropathy, but its regulatory mechanism is still unclear. This study is aimed at discussing the mechanisms underlying the effect of miRNA-155 on podocyte injury to determine its potential as a therapeutic target.

methodsCultured immortalized mouse podocytes and diabetic KK-Ay mice models were treated with a miR-155 inhibitor. Western blotting, real-time PCR, ELISA, immunofluorescence, and Luciferase reporter assay were used to analyze markers of inflammation cytokines and podocyte injury.

resultsmiRNA-155 was found to be highly expressed in serum and kidney tissue of mice with diabetic nephropathy and in cultured podocytes, accompanied by elevated levels of inflammatory factors. Inhibition of miRNA-155 can reduce proteinuria and ACR levels, diminish the secretion of inflammatory molecules, improve kidney function, inhibit podocyte foot fusion, and reverse renal pathological changes in diabetic nephropathy mice. Overexpression of miRNA-155

conclusionsThese findings strongly support the hypothesis that miRNA-155 inhibits podocyte inflammation and reduces podocyte injury through SIRT1 silencing. miRNA-155 suppression therapy may be useful for the management of diabetic nephropathy.

Indexed as

3' Untranslated RegionsAnimalsAnti-Inflammatory AgentsBinding SitesBlood GlucoseCytokinesDiabetic NephropathiesDisease Models, AnimalGene Expression Regulation, EnzymologicHEK293 CellsHumansInflammation MediatorsMiceMice, Inbred C57BLMicroRNAsOligonucleotides3' Untranslated RegionsAnti-Inflammatory AgentsBlood GlucoseCytokinesInflammation MediatorsMicroRNAsMirn155 microRNA, mouseOligonucleotidesSirt1 protein, mouseSirtuin 1

Identifiers

PMID33748285
PMCPMC7960039
OpenAlexW3134732438

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.