Evidence map›Paper›PMID 33748907›Full record

ArticleIn vitro cellular & developmental biology. Animal2021

Silencing MAP3K1 expression inhibits the proliferation of goat hair follicle stem cells.

Jinliang Ma, Jian Wang, Yunkui Feng, Liuming Zhang, Huiru Hu, Qiang Wang, Changjiang Chu, Jingwen Qu, Yanhu Wang, Yongjun Li

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Jinliang Ma *College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Jian Wang *College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Yunkui FengCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Liuming ZhangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Huiru HuCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Qiang WangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Changjiang ChuCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Jingwen QuCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Yanhu WangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Yongjun LiCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. liyj@yzu.edu.cn.ORCID http://orcid.org/0000-0002-7913-8533
Yangzhou University · CN

Funding

National Natural Science Foundation of China 31572355Priority Academic Program Development of Jiangsu Higher Education Institutions PAPD, 2014-134
6 · The paper itself

Abstract

The Yangtze River Delta White Goat is the only goat breed in the world that can produce superior-quality brush hair. Previous studies have shown that some genes are expressed differentially in the skin tissues between the goats produced superior-quality and normal-quality brush hair. Studies also have shown that different gene play varied roles in regulating the proliferation and apoptosis of hair follicle stem cells. However, the biological function of MAP3K1 (mitogen-activated protein kinase kinase kinase 1) gene in hair follicle stem cells is not fully understood. This study aims to investigate the role of MAP3K1 knockdown during the proliferation and apoptosis of hair follicle stem cells. RT-qPCR and Western blot were used to detect mRNA gene and protein expression level, CCK-8 and EdU assays were used to detect cell proliferation, and cell cycle and apoptosis were detected by flow cytometry. The results showed that the MAP3K1 expression level was significantly higher in the skin tissue of produced superior-quality brush hair than that in produced normal-quality brush hair. Moreover, functional studies indicated that si-MAP3K1 significantly inhibits the proliferation of hair follicle stem cells that came from a superior goat and promotes its apoptosis. Based on aforementioned assays, we speculated that MAP3K1 might play a regulatory effect in superior-quality brush hair traits.

Indexed as

AnimalsApoptosisCell ProliferationGene SilencingGoatsHair FollicleMAP Kinase Kinase Kinase 1Stem CellsMAP Kinase Kinase Kinase 1ApoptosisHair follicle stem cellsMAP3K1ProliferationYangtze River Delta White Goat

Identifiers

PMID33748907
OpenAlexW3196791185

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.