ArticlePlant biotechnology journal2021
CRISPR/Cas9-based discovery of maize transcription factors regulating male sterility and their functional conservation in plants.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 95 citations in OpenAlex.
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Genome-Wide Study of MYB Transcription Factors in Maize and Their Essential Roles in Male Fertility and Other Biological Processes.International journal of molecular sciences · 2026Article
- GmTGA9-GmDYT1 Regulates Anther Wall Development to Affect Male Fertility in Soybean.Plants (Basel, Switzerland) · 2026Article
- Maize Anther Development Involves Translated Open Reading Frames From 3' Untranslated Regions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Review
- The bHLH transcription factor OsbHLH148 negatively regulates rice tiller number and grain yield through an integrated transcriptional network.BMC genomics · 2026Article
- Biotechnological and genetic innovations to enhance sorghum adaptation under climate change.Frontiers in plant science · 2026Review
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Regulatory balance between ear rot resistance and grain yield and their breeding applications in maize and other crops.Journal of advanced research · 2025Review
- Resilience of Maize to Environmental Stress: Insights into Drought and Heat Tolerance.International journal of molecular sciences · 2025Review
- SPDC-HG: An accelerator of genomic hybrid breeding in maize.Plant biotechnology journal · 2025Article
- Genetic Analysis and Fine Mapping of Spontaneously Mutated Male Sterility Gene in Chinese Cabbage (Plants (Basel, Switzerland) · 2025Article
- The molecular mechanism and utilization of ZmMs7-mediated dominant nuclear sterility inFrontiers in plant science · 2025Article
- Integrating transcriptomic and proteomic analyses reveals impaired carbohydrate metabolism in tobacco cytoplasmic male sterility.Frontiers in plant science · 2025Article
- Plastid-localized ZmENR1/ZmHAD1 complex ensures maize pollen and anther development through regulating lipid and ROS metabolism.Nature communications · 2024Article
- A Critical Review of Recent Advances in Maize Stress Molecular Biology.International journal of molecular sciences · 2024Review
- Functional Mechanisms and the Application of Developmental Regulators for Improving Genetic Transformation in Plants.Plants (Basel, Switzerland) · 2024Review
- Molecular mechanisms controlling grain size and weight and their biotechnological breeding applications in maize and other cereal crops.Journal of advanced research · 2024Review
- Genome-Wide Identification and Characterization of Lignin Synthesis Genes in Maize.International journal of molecular sciences · 2024Article
- Genome-wide identification and analyses ofMolecular breeding : new strategies in plant improvement · 2024Article
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Authors and funding
16 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identifying genic male-sterility (GMS) genes and elucidating their roles are important to unveil plant male reproduction and promote their application in crop breeding. However, compared with Arabidopsis and rice, relatively fewer maize GMS genes have been discovered and little is known about their regulatory pathways underlying anther and pollen development. Here, by sequencing and analysing anther transcriptomes at 11 developmental stages in maize B73, Zheng58 and M6007 inbred lines, 1100 transcription factor (TF) genes were identified to be stably differentially expressed among different developmental stages. Among them, 14 maize TF genes (9 types belonging to five TF families) were selected and performed CRISPR/Cas9-mediated gene mutagenesis, and then, 12 genes in eight types, including ZmbHLH51, ZmbHLH122, ZmTGA9-1/-2/-3, ZmTGA10, ZmMYB84, ZmMYB33-1/-2, ZmPHD11 and ZmLBD10/27, were identified as maize new GMS genes by using DNA sequencing, phenotypic and cytological analyses. Notably, ZmTGA9-1/-2/-3 triple-gene mutants and ZmMYB33-1/-2 double-gene mutants displayed complete male sterility, but their double- or single-gene mutants showed male fertility. Similarly, ZmLBD10/27 double-gene mutant displayed partial male sterility with 32.18% of aborted pollen grains. In addition, ZmbHLH51 was transcriptionally activated by ZmbHLH122 and their proteins were physically interacted. Molecular markers co-segregating with these GMS mutations were developed to facilitate their application in maize breeding. Finally, all 14-type maize GMS TF genes identified here and reported previously were compared on functional conservation and diversification among maize, rice and Arabidopsis. These findings enrich GMS gene and mutant resources for deeply understanding the regulatory network underlying male fertility and for creating male-sterility lines in maize.
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