Evidence mapPaperPMID 33784258Full record

ArticleJCI insight2021

Perilipin 2 downregulation in β cells impairs insulin secretion under nutritional stress and damages mitochondria.

Akansha Mishra, Siming Liu, Joseph Promes, Mikako Harata, William Sivitz, Brian Fink, Gourav Bhardwaj, Brian T O'Neill, Chen Kang, Rajan Sah and 9 more

Open access · goldAbstract read
In one paragraph

Article in JCI insight, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
2.7field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. miRNA-642a-3p protects β cells from glucolipotoxicity.Molecular therapy. Nucleic acids · 2025
    Article
  6. Article
  7. Article
  8. Review
  9. Mechanisms of spinophilin-dependent pancreas dysregulation in obesity.American journal of physiology. Endocrinology and metabolism · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 5 institutions in 1 country.

Akansha MishraDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Siming LiuDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Joseph PromesDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Mikako HarataDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
William SivitzDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Brian FinkDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Gourav BhardwajDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Brian T O'NeillDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Chen KangDepartment of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
Rajan SahDepartment of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
Stefan StrackDepartment of Neuroscience and Pharmacology, Iowa Neuroscience Institute, University of Iowa, Iowa City, Iowa, USA.
Samuel StephensDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Timothy KingDepartment of Internal Medicine, Eastern Virginia Medical School, Norfolk, Virginia, USA.
Laura JacksonDepartment of Internal Medicine, Eastern Virginia Medical School, Norfolk, Virginia, USA.
Andrew S GreenbergObesity and Metabolism Laboratory, Jean Mayer United States Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, Massachusetts, USA.
Frederick Anokye-DansoDepartment of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
Rexford S AhimaDepartment of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
James AnkrumFraternal Order of Eagles Diabetes Research Center, University of Iowa, Iowa City, Iowa, USA.
Yumi ImaiDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
University of Iowa · USEastern Virginia Medical School · USJohns Hopkins University · USWashington University in St. Louis · USJean Mayer Human Nutrition Research Center on Aging · US

Funding

TARGETED MODULATION OF VASOPRESSIN IN BRAIN MAGNOCELLULAR NEURONS BY GENE THERAPYP30DK054759 · UNIVERSITY OF IOWA · 1998 to 2025
$7.4M
Washington University Nutrition Obesity Research CenterP30DK056341 · WASHINGTON UNIVERSITY · 1999 to 2025
$5.7M
SIGNAL TRANSDUCTION IN HUMAN ABDOMINAL AND GLUTEOFEMORAL ADIPOCYTESP30DK046200 · TUFTS MEDICAL CENTER · 1992 to 2005
$5.3M
CORE--GENETICS, GENOMICS, AND GENETIC EPIDEMIOLOGYP30DK072488 · UNIVERSITY OF MARYLAND BALTIMORE · 2005 to 2005
$1.0M
Biomarkers of Dietary Intake and Exposure Data Coordinating CenterU24DK129557 · DUKE UNIVERSITY · 2025 to 2025
$523k
Role of lipid droplet proteins in islet function in diabetes and obesityR01DK090490 · UNIVERSITY OF IOWA · 2025 to 2025
$472k
Regulation of Muscle Mitochondrial Protein Homeostasis by Insulin/IGF-1/FoxO SignalingI01BX004468 · VA · IOWA CITY VA MEDICAL CENTER · PI Brian Timothy O'Neill · 2023 to 2023
BLRD VA I01 BX004468BLRD VA I01 BX005072BLRD VA I01 BX005107NCRR NIH HHS S10 RR025439NICHD NIH HHS R21 HD098056NIDDK NIH HHS P30 DK046200NIDDK NIH HHS P30 DK054759NIDDK NIH HHS P30 DK056341NIDDK NIH HHS P30 DK072488NIDDK NIH HHS R01 DK090490NIDDK NIH HHS R01 DK106009NIDDK NIH HHS R01 DK108722NIDDK NIH HHS R01 DK123043NIDDK NIH HHS U24 DK097154NIDDK NIH HHS U24 DK129557NIDDK NIH HHS UC4 DK098085
6 · The paper itself

Abstract

Perilipin 2 (PLIN2) is a lipid droplet (LD) protein in β cells that increases under nutritional stress. Downregulation of PLIN2 is often sufficient to reduce LD accumulation. To determine whether PLIN2 positively or negatively affects β cell function under nutritional stress, PLIN2 was downregulated in mouse β cells, INS1 cells, and human islet cells. β Cell-specific deletion of PLIN2 in mice on a high-fat diet reduced glucose-stimulated insulin secretion (GSIS) in vivo and in vitro. Downregulation of PLIN2 in INS1 cells blunted GSIS after 24-hour incubation with 0.2 mM palmitic acid. Downregulation of PLIN2 in human pseudoislets cultured at 5.6 mM glucose impaired both phases of GSIS, indicating that PLIN2 is critical for GSIS. Downregulation of PLIN2 decreased specific OXPHOS proteins in all 3 models and reduced oxygen consumption rates in INS1 cells and mouse islets. Moreover, we found that PLIN2-deficient INS1 cells increased the distribution of a fluorescent oleic acid analog to mitochondria and showed signs of mitochondrial stress, as indicated by susceptibility to fragmentation and alterations of acyl-carnitines and glucose metabolites. Collectively, PLIN2 in β cells has an important role in preserving insulin secretion, β cell metabolism, and mitochondrial function under nutritional stress.

Indexed as

AnimalsCarnitineDiet, High-FatDown-RegulationGlucoseHumansInsulin-Secreting CellsInsulin SecretionIn Vitro TechniquesIslets of LangerhansLipid DropletsMiceMice, KnockoutMitochondriaOleic AcidOxidative PhosphorylationacylcarnitineCarnitineGlucoseOleic AcidPerilipin-2DiabetesEndocrinologyIslet cellsMetabolism

Identifiers

PMID33784258
PMCPMC8262280
OpenAlexW3146062416

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.