ArticleProceedings of the National Academy of Sciences of the United States of America2021
A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 79 citations in OpenAlex.
- The endothelial scavenger receptorbioRxiv : the preprint server for biology · 2026Article
- Functional genomics analysis of developing zebrafish and human endoderm reveals highly conservedGenome research · 2026Article
- Balancing act: how Apelin tunes vascular and haemogenic identities.EMBO reports · 2026Article
- Dynamic single-cell transcriptomics reveals lsamp-guided neural network formation in male S. japonicum driving female reproduction.Nature communications · 2026Article
- Ace2 safeguards embryonic hematopoietic stem and progenitor cell production by restraining Nlrp3-mediated pyroptosis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Chromatin Remodeler Chd8 Regulates Hematopoietic Stem and Progenitor Cell Survival and Differentiation During Zebrafish Embryogenesis.International journal of molecular sciences · 2025Article
- Tead1a Initiates Transcriptional Priming Through the TEAD1a/YAP-Notch1-Spi1/Cebpα Axis to Promote Neutrophil Fate.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The axillary lymphoid organ is an external, experimentally accessible immune organ in the zebrafish.The Journal of experimental medicine · 2025Article
- HemAtlas: A Multi-omics Hematopoiesis Database.Genomics, proteomics & bioinformatics · 2025Article
- [Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2025Article
- Mutational cooperativity of RUNX1::RUNX1T1 isoform 9a and oncogenic NRAS in zebrafish myeloid leukaemia.Biology open · 2024Article
- The heart is a resident tissue for hematopoietic stem and progenitor cells in zebrafish.Nature communications · 2024Article
- Reprogramming of 3D genome structure underlying HSPC development in zebrafish.Stem cell research & therapy · 2024Article
- Development of an Automated Morphometric Approach to Assess Vascular Outcomes following Exposure to Environmental Chemicals in Zebrafish.Environmental health perspectives · 2024Article
- A Mettl16/mThe EMBO journal · 2024Article
- Distinct roles of core autophagy-related genes in zebrafish definitive hematopoiesis.Autophagy · 2024Article
- Nlrc3 signaling is indispensable for hematopoietic stem cell emergence via Notch signaling in vertebrates.Nature communications · 2024Article
- Exposure to Nanoplastics Cause Caudal Vein Plexus Damage and Hematopoietic Dysfunction by Oxidative Stress Response in ZebrafishInternational journal of nanomedicine · 2024Article
- Fishing Innate Immune System Properties through the Transcriptomic Single-Cell Data ofBiology · 2023Review
- Widespread sex dimorphism across single-cell transcriptomes of adult African turquoise killifish tissues.Cell reports · 2023Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During vertebrate embryogenesis, fetal hematopoietic stem and progenitor cells (HSPCs) exhibit expansion and differentiation properties in a supportive hematopoietic niche. To profile the developmental landscape of fetal HSPCs and their local niche, here, using single-cell RNA-sequencing, we deciphered a dynamic atlas covering 28,777 cells and 9 major cell types (23 clusters) of zebrafish caudal hematopoietic tissue (CHT). We characterized four heterogeneous HSPCs with distinct lineage priming and metabolic gene signatures. Furthermore, we investigated the regulatory mechanism of CHT niche components for HSPC development, with a focus on the transcription factors and ligand-receptor networks involved in HSPC expansion. Importantly, we identified an endothelial cell-specific G protein-coupled receptor 182, followed by in vivo and in vitro functional validation of its evolutionally conserved role in supporting HSPC expansion in zebrafish and mice. Finally, comparison between zebrafish CHT and human fetal liver highlighted the conservation and divergence across evolution. These findings enhance our understanding of the regulatory mechanism underlying hematopoietic niche for HSPC expansion in vivo and provide insights into improving protocols for HSPC expansion in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.