ArticleBiotechnology for biofuels2021
Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris.
Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
- Enhanced secretion of thermostable phytases from Myceliophthora thermophila by Komagataella phaffii.Microbial cell factories · 2026Article
- High-throughput yeast engineering in biofoundries: towards autonomous and scalable synthetic biology.FEMS yeast research · 2026Review
- Thermostable Esterase from ThermophilicGenes · 2025Article
- Recent advances in phytase thermostability engineering towards potential application in the food and feed sectors.Food science and biotechnology · 2025Review
- Engineering of the Phytase YiAPPA to Improve Thermostability and Activity and Its Application Potential in Dephytinization of Food Ingredients.Journal of microbiology and biotechnology · 2024Article
- Improved Thermal Stability of a Novel Acidophilic Phytase.Journal of microbiology and biotechnology · 2024Article
- The application of protease in aquaculture: Prospects for enhancing the aquafeed industry.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Review
- Review
- COMicrobial cell factories · 2024Article
- Effect ofMolecules (Basel, Switzerland) · 2023Article
- Industrial Production of Proteins withBiomolecules · 2023Review
- The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii.Biotechnology for biofuels and bioproducts · 2022Article
- Identifying immunodominant multi-epitopes from the envelope glycoprotein of the Lassa mammarenavirus as vaccine candidate for Lassa fever.Clinical and experimental vaccine research · 2022Article
- Enhancing the Thermostability of Phytase to Boiling Point by Evolution-Guided Design.Applied and environmental microbiology · 2022Article
- Disulfide Bond Engineering for Enhancing the Thermostability of the Maltotetraose-Forming Amylase fromFoods (Basel, Switzerland) · 2022Article
- Comparative transcriptomic analysis-based identification of the regulation of foreign proteins with different stabilities expressed inFrontiers in microbiology · 2022Article
- Characterisation of a soil MINPP phytase with remarkable long-term stability and activity from Acinetobacter sp.PloS one · 2022Article
- Bioengineered textiles with peptide binders that capture SARS-CoV-2 viral particles.Communications materials · 2022Article
- Enhanced Thermostability of D-Psicose 3-Epimerase fromInternational journal of molecular sciences · 2021Article
- Improvement of the Stability and Activity of an LPMO Through Rational Disulfide Bonds Design.Frontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundPhytases are widely used commercially as dietary supplements for swine and poultry to increase the digestibility of phytic acid. Enzyme development has focused on increasing thermostability to withstand the high temperatures during industrial steam pelleting. Increasing thermostability often reduces activity at gut temperatures and there remains a demand for improved phyases for a growing market.
resultsIn this work, we present a thermostable variant of the E. coli AppA phytase, ApV1, that contains an extra non-consecutive disulfide bond. Detailed biochemical characterisation of ApV1 showed similar activity to the wild type, with no statistical differences in k
conclusionsOverall, the results show that protein engineering for enhanced enzymatic properties like thermostability may result in folding complexity and decreased production in microbial systems. Hence parallel development of improved production strains is imperative to achieve the desirable levels of recombinant protein for industrial processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.