Evidence map›Paper›PMID 33796127›Full record

ArticleFrontiers in plant science2021

Comprehensive Characterization and Validation of Chromosome-Specific Highly Polymorphic SSR Markers From Pomegranate (

Prakash Goudappa Patil, Nripendra Vikram Singh, Abhishek Bohra, Keelara Puttaswamy Raghavendra, Rushikesh Mane, Dhananjay M Mundewadikar, Karuppannan Dhinesh Babu, Jyotsana Sharma

Abstract read
In one paragraph

Article in Frontiers in plant science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Development of Genome-Wide SSR Markers inInternational journal of molecular sciences · 2025
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  6. Article
  7. Article
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  14. Genome skimming-based simple sequence repeat (SSR) marker discovery and characterization inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021
    Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Prakash Goudappa PatilICAR-National Research Centre on Pomegranate, Solapur, India.
Nripendra Vikram SinghICAR-National Research Centre on Pomegranate, Solapur, India.
Abhishek BohraICAR-Indian Institute of Pulses Research, Kanpur, India.
Keelara Puttaswamy RaghavendraICAR-Central Institute for Cotton Research, Nagpur, India.
Rushikesh ManeICAR-National Research Centre on Pomegranate, Solapur, India.
Dhananjay M MundewadikarICAR-National Research Centre on Pomegranate, Solapur, India.
Karuppannan Dhinesh BabuICAR-National Research Centre on Pomegranate, Solapur, India.
Jyotsana SharmaICAR-National Research Centre on Pomegranate, Solapur, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The simple sequence repeat (SSR) survey of 'Tunisia' genome (296.85 Mb) identified a total of 365,279 perfect SSRs spanning eight chromosomes, with a mean marker density of 1,230.6 SSRs/Mb. We found a positive trend in chromosome length and the SSR abundance as marker density enhanced with a shorter chromosome length. The highest number of SSRs (60,708) was mined from chromosome 1 (55.56 Mb), whereas the highest marker density (1,294.62 SSRs/Mb) was recorded for the shortest chromosome 8 (27.99 Mb). Furthermore, we categorized all SSR motifs into three major classes based on their tract lengths. Across the eight chromosomes, the class III had maximum number of SSR motifs (301,684, 82.59%), followed by the class II (31,056, 8.50%) and the class I (5,003, 1.37%). Examination of the distribution of SSR motif types within a chromosome suggested the abundance of hexanucleotide repeats in each chromosome followed by dinucleotides, and these results are consistent with 'Tunisia' genome features as a whole. Concerning major repeat types, AT/AG was the most frequent (14.16%), followed by AAAAAT/AAAAAG (7.89%), A/C (7.54%), AAT/AAG (5.23%), AAAT/AAAG (4.37%), and AAAAT/AAAAG (1.2%) types. We designed and validated a total of 3,839 class I SSRs in the 'Tunisia' genome through electronic polymerase chain reaction (ePCR) and found 1,165 (30.34%) SSRs producing a single amplicon. Then, we selected 906 highly variable SSRs (> 40 nt) from the ePCR-verified class I SSRs and

Indexed as

chromosomegenomehypervariable SSRpolymorphismpomegranate

Identifiers

PMID33796127
PMCPMC8007985

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.