Evidence mapPaperPMID 33845779Full record

ArticleBMC cardiovascular disorders2021

Experimental parameters and infarct size in closed chest pig LAD ischemia reperfusion models; lessons learned.

Max J M Silvis, Gerardus P J van Hout, Aernoud T L Fiolet, Mirthe Dekker, Lena Bosch, Martijn M J van Nieuwburg, Joyce Visser, Marlijn S Jansen, Leo Timmers, Dominique P V de Kleijn

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In one paragraph

Article in BMC cardiovascular disorders, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Guidelines for in vivo mouse models of myocardial infarction.American journal of physiology. Heart and circulatory physiology · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Max J M SilvisDepartment of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
Gerardus P J van HoutDepartment of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
Aernoud T L FioletDepartment of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
Mirthe DekkerDepartment of Cardiology, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.
Lena BoschDepartment of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
Martijn M J van NieuwburgDepartment of Experimental Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
Joyce VisserDepartment of Experimental Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
Marlijn S JansenDepartment of Experimental Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
Leo TimmersDepartment of Cardiology, St. Antonius Hospital, Nieuwegein, The Netherlands.
Dominique P V de KleijnDepartment of Vascular Surgery, University Medical Center Utrecht, Heidelberglaan 100, 3508 GA, Utrecht, The Netherlands. dkleijn@umcutrecht.nl.ORCID 0000-0003-2714-2140

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPreclinical models that resemble the clinical setting as closely as possible are essential in translating promising therapies for the treatment of acute myocardial infarction. Closed chest pig left anterior descending coronary artery (LAD) ischemia reperfusion (I/R) models are valuable and clinically relevant. Knowledge on the influence of experimental design on infarct size (IS) in these models is a prerequisite for suitable models. To this end, we investigated the impact of several experimental features (occlusion and follow-up time and influence of area at risk (AAR)) on IS.

methodsA total of fifty-one female Landrace pigs were subjected to closed chest LAD balloon occlusion and evaluated in three substudies with varying protocols. To assess the relationship between time of occlusion and the IS, 18 pigs were subjected to 60-, 75- and 90 min of occlusion and terminated after 24 h of follow-up. Influence of prolonged follow-up on IS was studied in 18 pigs after 75 min of occlusion that were terminated at 1, 3 and 7 days. The relation between AAR and IS was studied in 28 pigs after 60 min of occlusion and 24 h of follow-up. The relation between VF, number of shocks and IS was studied in the same 28 pigs after 60 min of occlusion.

resultsIncreasing occlusion time resulted in an increased IS as a ratio of the AAR (IS/AAR). This ranged from 53 ± 23% after 60 min of occlusion to 88 ± 2.2% after 90 min (P = 0.01). Increasing follow-up, from 1 to 3 or 7 days after 75 min of occlusion did not effect IS/AAR. Increasing AAR led to a larger IS/AAR (r

conclusionsThese experiments describe the association of occlusion time, follow-up duration, AAR and VF with IS in closed chest pig LAD I/R models. These results have important implications for future I/R studies in pigs and can serve as a guideline for the selection of appropriate parameters and the optimal experimental design.

Indexed as

AnimalsBalloon OcclusionDisease Models, AnimalElectric CountershockFemaleMyocardial InfarctionMyocardial ReperfusionMyocardial Reperfusion InjuryMyocardiumSus scrofaTime FactorsVentricular FibrillationIschemia/reperfusion injuryLADMyocardial infarctionPig model

Identifiers

PMID33845779
PMCPMC8042863

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.